ONPG Solution preparation?

24 March 2019 1 7K Report

I am using ONPG as a substrate to check the enzyme activity of the beta-galatosidase. For that I need to prepare a solution of 5 mg/ml ONPG accurately. Also I've prepared a 100mM pH = 7.3 phosphate buffer.

When dissolving ONPG in the buffer it does not completely dissolve and I was asked to heat the solution gently to dissolve the powder. In that case what is the optimum temperature to heat the solution without degrading the substrate?

Thanks in advance.

Kasun D. Abeyrathne

Simon Dingsdag

Thank you for taking your time on answering the question.

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