Nuclear Matrix: After DNAse digestion and high salt wash of isolated nuclei, the DNAse gets stuck in the nuclear scaffold. Why?

More Ashish Bihani's questions See All

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Dmitry Guschin

Hi Ashish, what are you going to investigate? Could you simply heat-inactivate DNAse1? If you next step is some sort of PCR, heat inactivation should not be a problem.

Ashish Bihani

Hi. Thanks for response.

No. I want to look at the ultrastructure of the residual complex. The activity is not a problem. DNAse could cause formation of faux structures.