09 December 2019 3 543 Report

Hi all,

I have been trying to do some cloning recently. When I am doing the ligation, I have 1 control: No insert control. In the no insert control, I did not put insert into the vector, which is also treated with Alkaline Phosphatase. Therefore, in this control, there should have no colonies formed. However, there are almost as much colonies formed as the true ligation, i.e. with both insert and vector.

Therefore, is there reason why and how can I avoid this problem?

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