Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that were then crossed. For future planning, is it reasonable to expand a Cas9 KI+/+ Cre+/+ colony and then breed these animals to a Cas9 KI+/+ Cre-/- mice? Then all pups will be Cas9+/+ Cre-/+. The experiment require a large quantity of male mice.

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