Mathew Chan

5 Questions 5 Answers 0 Followers

Questions related from Mathew Chan

What should I do with low transformation efficiency with DH5a?

Hi all, I am currently trying to introduce a mutant gene and sub clone the wild type gene from pcDNA3.0 into pcDNA3.1. After each gel purification, from PCR product to enzyme digestion, I have...

06 January 2020 8,857 4 View

Why are there residue RNA in the mini prep?

After I did the mini prep, the concentration is about 300ng/ul and A260/280 is 2.13. However, no plasmid can be seen after I loaded 5ul to run a gel and there is a what I believe a RNA...

27 December 2019 3,255 3 View

Why no bands in gel electrophoresis after extracting the plasmid from mini prep?

Hi all, I have been trying to do cloning recently. After lots of effort, I finally come to do the mini prep with iNTRON kit. When we shake the colony in 5 ml of LB with 5ul of ampicillin. There...

23 December 2019 882 3 View

No insert control grows colonies even with CIP treatment for the vector?

Hi all, I have been trying to do some cloning recently. When I am doing the ligation, I have 1 control: No insert control. In the no insert control, I did not put insert into the vector, which is...

09 December 2019 574 3 View

How to create a amino acid and nucleotide sequence alignment graph?

I am trying to create a graph as shown below with my gene and protein of interest I found on NCBI, which are NM_001904.4 and NP_001895.1 respectively. As my protein consists of 781 aa, I am...

26 July 2019 7,885 2 View