Hello,
Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)?
As far as I understand it there is no specific sequence on the plasmid required foe packaging to work, it just depends on the size.
Is it correct?
We are currently using pGata backbone for this and it works well.
Thank you for your help