Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Ideas for Discussion of a Case-study analysis?

I am currently researching the demotivating factors and remotivating factors of high school students in my country. My study only has 13 participants, and I groups them into 3 groups depending on...

02 December 2020 2,288 3 View

About cross-case analysis?

Currently, I have finished interviewing my high school students to learn about their demotivating and remotivating factors. I'm trying to use case-study method to analysis data. In my...

17 November 2020 7,989 2 View

Dear All, could you please show me how should I do to open SIR-C compressed image with ENVI 5.3 or ArcGIS 10.4 softwares?

I would like to apply a remote sensing processing in this file for a structural geologic feature study. The compressed file name: PR48120.tar The three unzipped files which come from PR48120.tar...

23 September 2020 4,924 2 View

About researching EFL learning demotivation and Straussian grounded theory?

Currently, I am conducting a MA dissertation by using Strauss and Corbin's Grounded Theory. My topic is researching EFL learning demotivation of high school students (16 to 18 years old). My main...

13 July 2020 6,773 4 View

What kind of optimization is done through genetic algorithm in architecture?

Difficulties and types of optimization

11 May 2020 6,177 1 View

Which Grounded Theory should I use?

I am a novice researcher who is writing my dissertation. My topic is high school students' demotivation and their coping strategies in my country. Currently, I am thinking about using Straussian...

15 February 2020 2,243 3 View

How can I convert a pre-trained CNN model in Caffe to Keras?

Hi everybody, I would like to convert a pre-trained Caffe model to Keras format. If I understood correctly I have to extract the output shape and the weights of the Caffe model, build a model in...

22 May 2019 112 2 View

Command line for results in autodock4?

Dear professors, Could you please tell me the command line to transfer dlg results file into pdbqt files in COMMAND PROMT? Im very hopeful for your advises. Kind regards.

18 May 2019 4,205 2 View

Add charges to Zn in protein?

I am now using Autodock Tools and Autodock 4 to figure out the interaction of ligand-protein. However, there is a problem I have to face now is that the Zn in the protein has 0 charge instead of...

15 May 2019 4,171 5 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View