Typical neutralization buffer used for Protein A/G affinity separations is 1 M Tris/HCl pH 9.0
Any alternate buffer for 1 M Tris/HCl ?
The point of the neutralization buffer is to bring the pH back to near-neutral after the acidic elution step. You can use any basic buffer. Experiment by mixing the buffer solution with the elution reagent and testing the resulting pH.
Yes, thanks for your suggestion. will Try!
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