13 Questions 20 Answers 0 Followers
Questions related from Manohar Raju
Hi All, I do have a single-stranded oligonucleotide of 100 - 150 bases synthesized and the sequence is not known. How would I proceed to know the sequence .
05 February 2021 5,767 3 View
Hi all, I am trying to immobilize Antibody by DTSSP on a gold substrate, The DTSSP has a center disulfide. Most of the research articles have not reported the reducing of DTSSP...
27 November 2019 8,866 1 View
Hi, In most of the DNA biosensor experiments and articles they have used MCH (6-Mercaptohexanol) as a blocking agent, to orient DNA 90o and to remove non-specifically bound from the...
28 May 2019 3,116 1 View
Hi All, How one can quantify the oligonucleotides for 10 - 100pM range, i had used nanodrop to quantify but ended up with the result same as blank. The oligo's had dispensed in...
21 December 2018 8,703 2 View
Hi, Am using Electrochemical workstation 660E, Am not able to get X and Y axis values, have tried from rebooting the system to deleting the regedit includes deleting old data. if someone faced...
15 June 2018 7,247 4 View
Hi, How difficulty is to remove the AuNp's coated on to the Gold electrode surface, can it be removed completely 100% ? Can one remove the AuNp's by Polishing with Alumina Powder and...
12 April 2018 9,455 3 View
Hi, I have been working on Bacillus thuringiensis, when i amplify the Cry1 gene using genomic DNA i usually get a positive result. i need to know whether these Cry gene's are Genomic or...
26 October 2017 4,370 4 View
Hi Everyone, Am looking for a Methods / Protocols for Electrochemical Biosensors, can some one shed the light on these aspects,since am new to Electrochemical biosensors , my aim is to build a...
15 September 2017 2,391 3 View
Hi Everyone, i have a Sequence of 16s rRNA, when i tried to generate CAP- Contig using BioEdit, am getting Multiple Contigs for a particular strain, but when i tried with some other strain am...
11 January 2017 610 2 View
Am using an ~ 6 years old Primers now, and am not able to get any amplicons, can this be due to degradation of nucleotides over time ? .
17 June 2016 9,364 9 View
Nanotechnology on Proteins for Larvicial activity and the equipments used for Nanotechnology.
16 May 2016 4,205 3 View
1.What is the Colony Morphology of Bacillus thuringiensis, If possible post a Pic of culture plate. 2. How does Cry Proteins and Spores look like after Staining. 3.Better Method to isolate DNA...
06 April 2016 6,514 3 View
Hi Everyone, I am trying to check for Gene Expression in one of the Bacteria(prokaryote), so i had planned for RNA-Sequencing i.e. Transcriptome Analysis.So, 1.Is it possible to isolate m-RNA...
01 January 1970 7,970 5 View
