One of my friend got pricked with the same needle which he had used for cell culture and drop of blood also came. So i just wanted to know how harmful is it? Does someone have any idea regarding this?
It depends on what type of cell culture you have used while needleprick happened.
In general cell culture or cell lines wont do much on the blood contamination, possibliligy for cancer inducing is extremely very low,
To be positive, dont worry about it, but try to keep your body at normal temperature, if febrile condition, consult your doctor with the specification of cell line prick you used.
Otherwise no issues. as per my concern. Hope you cleaned it with alcohol once the needle prick occured/or first aid as per your lab protocol.
Been there, done that!
'Your friend' needs to see the health and safety person in your department. He should try and see what type of cells he was working with. Potentially infectious cells? And what species did the cells came from? My cells were immortalized human leukaemic cell lines so I had was OK. but it really depends on the cell type. There must be some health and safety documents on the website where he purchased the cells from.
Hope it helps.
Yasar
Is it a human cell culture? Any way you should take care of these things. Still i don't think there is anything to worry about.
It also happened to me when I started working in cell culture but nothing happened to me. There could be an issue if you were working with some viral culture, then there are chances of infection, otherwise if you was working only with a cell line then there is as such nothing to worry. On the safe side, report the injury in your lab or institution note book for record.
Report the incident to your lab or institution. As long as the cell culture was free of bacteria or viruses, there should not be any problem.
First thing to do is to report the incident to the H&S person in the Lab. But i also think unless its a really infectious cell line then there is nothing to worry about.
Needlesticks shouldn't happen, period. You should assume that any cells or culture you work with is infected with a harmful pathogen and work in such a manner that you never place yourself in a position to innoculate yourself.
Doing 'dry runs' with needles allows you to work out the safest possible series of actions you should perform. When we perform injections of cancer cell cultures into the flanks of mice, we have already practiced how we will hold the mice and how we will inject, so we don't needlestick.
Talk to someone with more experence, plan your movement and practice, practice, practice
I totally agree that employee health needs to be informed and some standard lab tests need to be done. However, I do not understand why needle was used for cell culture unless the needle was used to draw blood from human or animal. Without any detail it will be hard to say it will be safe or not. Though it will be extremely rare but there is a possibility that malignat cells can be innoculate to the recepient. Also, if hepatitis B and C is involved then the chance to get infected will be very high. I am not too worry about HIV which has a very low infectivity.
In our lab I use a sterile syringе and a 20G needle to break down clumps of HepG2 cells after trypsinization (they tend to be very clumpy). As already noted, before I stared doing it, I practiced several times with water to avoid situations like that.
Moreover, even if the cell line is derived from a human, it expresses surface antigens that are recognized by our immune system and the cells will be killed.
The danger mostly comes if this was a primary human-derived cell line (preadipocytes, muscle biopsies, etc) - the donor could have had undetected HIV, hepatitis C and whatever else you like.
Thank you so much for all the responses. The cell line was HepG2. After reading all the comments, i assume that we don't need to worry.
While exposure to blood-borne pathogens is the major concern from an accidental needle stick, any puncture wound carries the potential for tetanus. Generally, a tetanus booster shot is given every 10 years. If it was more than 10 years ago,since your last tetanus shot, then you might want to ask your doctor about it, especially if you continue to be at risk for puncture wounds.
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