As we all know that one of the main isolation techniques was preparative TLC, where we first marked the TLC with pencil and then load sample in bulk followed by elution using optimized mobile phase. The spots then encircled using pencils and scratched using sterilized knife. This silica will then used for extraction using suitable solvent.
I need reference articles for this protocol. Please help me in collecting the same.
An Introduction to Planar Chromatography and Its Application to Natural Products Isolation
http://link.springer.com/protocol/10.1007/978-1-61779-624-1_6
Something like this one?
http://www.ncbi.nlm.nih.gov/pubmed/24377738
Just google "Preparative TLC natural products"
Dear Rajeev, it would be good to know chemical structure (even roughly) and properties of your metabolites. Then you can start looking for methods that were used for isolation of similar molecules.
But I would simply start by assessing the polarity/acidity/basicity/charge of molecules of interest and matrix. Then decide on best isolation procedure. You might also consider liquid/liquid extraction, precipitation, ion exchange or some preparative FLASH chromatography. If you want to go with prep TLC, I would try various mixtures of solvents like hexane, toluene, dichloromethane, acetone, methanol, acetic acid, even water (but not too much it dissolves nonmodified silica) and run a few small TLCs to see. Extracting adsorbed molecules from silica again depends on character of your metabolites. If you dont know you wil unfortunately have to experiment :).
Cheers, Petr
Dear Kit, Can u provide me copy of that article as I don't have access for that article.
Dear Yoram, I just need TLC articles where by using TLC(not HPLC or HPTLC) scientists had isolated secondary metabolites.
I tried google with the similar keywords like this, but unable to get a high quality article.
Thanks a lot Kit Leong Cheong for sending me this article. Is there any other references available. Please provide me.
Dear Ali Abdil Razzaq Muhammed Noori Aldallal ,
Thanks a lot for sending these articles. Actually I am looking for articles where isolation technique using TLC has been given in details. In these papers they stressed more on normal TLC technique as well as bioautography. I need purification procedure using TLC.
Dear Rajeev,
A number of papers mentioning preparative TLC method of isolation of secondary metabolites are available on internet. A few are attached for your reference.
Dear Yogesh Sir,
Thanks a lot for providing me these articles. in These articles also, how authors do the marking on TLC is not available. Which material they are preferring for marking purpose.
Dear Rajeev,
Pl find attached some more papers on this aspect that may solve your purpose.
Thanks Yogesh Sir for providing these articles. I am highly obliged.
Dear Dr. Khedr,
At present, we don't have semi-preparative HPLC-UV in our lab, so need to manage with TLC only. So if possible, plz send me the reference articles.
Regards
Rajeev K Singla
Dear Sir,
Please find attached an article related to Preparative TLC concept. Hope, this will solve your purpose.
Paper send by Amrit Kumar singh Is good to know use preparative TLC. but if after TLC if u use column chromatography you will get better result and good marker compound
tHE clhp SEMI PREPARTIF IS VERY EXPENSIFµ
YOU CAN USE use column chromatography WITH SMALl DIAMETRE OR
THE ccm SEMI AUTOMATIC/ PLATE CIRCULAR CHROMATOTRON IS VERY GOOD FOR SPERATION AND NOT EXPENSIF
MAHROUZ MOSTAFA
MARRAKECH
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