How to determine the false positive sequencing result?

Hello Researchers. I am conducting a study on the determination of WNV on mosquito samples. I run nested PCR. Bands were shown to almost all samples during carrying out gel electrophoresis. After...

17 January 2021 3,263 11 View

How can we increse the osmotic pressure of a solution while maintaining the electrical conductivity of the solution?

in other words what kind of salts increase the osmotic pressure with the same salt content. for example 1g of NaCl or 1 gr of Na2SO4 or KCl or ....

25 January 2019 2,610 3 View

Dental decision support systems by Image Processing and Data Mining?

Intelligent Therapist Designer for Dentistry with Data mining and image processing

18 January 2019 9,288 3 View

Surface treatment and finishing for BiFeO3?

Hello everyone, I want to do surface treatment for my sol-gel synthesized BiFeO3. For this purpose if anyone have a kind suggestions regarding methodology that how to do and which way is better...

26 December 2018 7,295 7 View

How to check valence state of Cr using UV vis spectroscopy?

I want to ask about how to get the accurate valence statr of Cr in solution form? As from XPS I found it is +3 but somebody ask that Cr+3 can not be attached on material in acidic solution. So ask...

10 December 2018 2,502 3 View

Can we measure the employee performance by asking employee directly instead of their managers? or if anybody have measurement instrument please Share?

I want to study the impact of Social media usage on employee's performance so therefore i need measurement instrument for employee's performance if anybody can help me, or wants to give me some...

30 June 2018 3,071 10 View

Can mosquitoes be homogenized with mechanical homogenizer without beads?

I am a master student under virology program. Currently, I am working on determination of virus among mosquitoes population . Based on previous study, most of them used bead-based homogenization...

30 May 2018 6,836 15 View

Do I have to get consents for thousands of samples in my ethical approved retrospective study ? What about passed away poeple ?

I have an ethical approved project to conduct retrospective and prospective studies on sickle cell disease . I need the old DEXA scans , MRI , bone scans , CBC , HPLC and biochemistry results for...

05 June 2017 2,702 2 View

If two powder xrd patterns are the same, can we conclude that they are the same compounds?

We have prepared some catalysts with some modifications, but the core structure is the same. we have recorded the Xrd patterns. some of them are the same. It should be mentioned that the melting...

29 May 2017 6,929 8 View

Minimum current that can charge Li battery?

31 March 2016 4,964 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How can Gamma Ray API units be converted to CPS (counts per second)?

Oil and gas companies run gamma ray tool and take readings in API units while Gamma ray is also noted in cps, so how these units can be converted into each other?

24 February 2021 7,545 2 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

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22 February 2021 9,932 11 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View