How to determine the false positive sequencing result?

Hello Researchers. I am conducting a study on the determination of WNV on mosquito samples. I run nested PCR. Bands were shown to almost all samples during carrying out gel electrophoresis. After...

17 January 2021 3,263 11 View

How can we increse the osmotic pressure of a solution while maintaining the electrical conductivity of the solution?

in other words what kind of salts increase the osmotic pressure with the same salt content. for example 1g of NaCl or 1 gr of Na2SO4 or KCl or ....

25 January 2019 2,610 3 View

Dental decision support systems by Image Processing and Data Mining?

Intelligent Therapist Designer for Dentistry with Data mining and image processing

18 January 2019 9,288 3 View

Surface treatment and finishing for BiFeO3?

Hello everyone, I want to do surface treatment for my sol-gel synthesized BiFeO3. For this purpose if anyone have a kind suggestions regarding methodology that how to do and which way is better...

26 December 2018 7,295 7 View

How to check valence state of Cr using UV vis spectroscopy?

I want to ask about how to get the accurate valence statr of Cr in solution form? As from XPS I found it is +3 but somebody ask that Cr+3 can not be attached on material in acidic solution. So ask...

10 December 2018 2,502 3 View

Does anyone have an idea to fabricate a high temperature / high pressure reference electrode?

07 July 2018 4,484 8 View

Can we measure the employee performance by asking employee directly instead of their managers? or if anybody have measurement instrument please Share?

I want to study the impact of Social media usage on employee's performance so therefore i need measurement instrument for employee's performance if anybody can help me, or wants to give me some...

30 June 2018 3,071 10 View

Can mosquitoes be homogenized with mechanical homogenizer without beads?

I am a master student under virology program. Currently, I am working on determination of virus among mosquitoes population . Based on previous study, most of them used bead-based homogenization...

30 May 2018 6,836 15 View

Do I have to get consents for thousands of samples in my ethical approved retrospective study ? What about passed away poeple ?

I have an ethical approved project to conduct retrospective and prospective studies on sickle cell disease . I need the old DEXA scans , MRI , bone scans , CBC , HPLC and biochemistry results for...

05 June 2017 2,702 2 View

If two powder xrd patterns are the same, can we conclude that they are the same compounds?

We have prepared some catalysts with some modifications, but the core structure is the same. we have recorded the Xrd patterns. some of them are the same. It should be mentioned that the melting...

29 May 2017 6,929 8 View

Can one use just 250 micron sieve & still get a good number of mycorrhizal spores from the soil?

In a situation where one cannot get the appropriate & separate levels of sieve for mycorrhizal survey work, can just one level of sieve give good results & spore counts?

13 February 2021 3,760 2 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

Loss of biodiversity its impact on terrestrial ecosystem?

I need research papers with problem statement as well as research questions in respect of the above mentioned topics

04 February 2021 2,240 15 View

How to preserve wet fungus spore ?

I need to preserve broth culture of wet fungus spore for my further research. Can any one suggest me any solution.

18 January 2021 4,756 2 View

Can anyone identify this fungus/mushroom from China?

The pictures were taken in Guishan Mts in Yunnan. The fungus is ca. 10-12 cm high.

07 January 2021 6,639 10 View

Could one suggest a common formula for Penicillium Chrysogenum spore counting by spectroscopy? and also its nm?

I am working with this strain but I do not have the formula for spore counting. please just suggest a spectrophotometric method.

05 January 2021 4,308 3 View

Is this method mentioned for sporulation assessment reliable ?

I had come across a protocol to assess and score the sporulation of fungi, where they has taken out a 5 mm fungal disc and transferred to 5ml sterile dist.water and spore count was recorded under...

05 January 2021 9,468 1 View

Can forward and reverse sequences of PCR products be entirely different from each other?

Dear All, The forward and reverse sequences obtained from the PCR products are not identical at all. Can I still make the contigs for doing BLASTn? Individually both sequences are showing the...

22 December 2020 5,280 5 View

CFU treatment log reduction without control?

Hello all, I am trying to create a graph on excel to express -log10 reduction of treatment groups and CFU spores using the equation -log10(final concentration/initial). However, one of my groups...

28 November 2020 6,074 3 View

How can i get nonsporulating bacterial bacillus subtillis strain spoIIE without antibiotic growth conditions ?

10 November 2020 7,168 4 View