Any modifications of culture conditions for Nalm6 cells besides RPMI + 10% FBS?
I'm using a InVia Renishaw Raman microscope system (785nm wavelength, 600l/mm grating density) to acquire Raman images on human brain samples, at 50um resolution. The samples are placed on MgF2...
30 July 2024 8,771 3 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
Hi guys, I am trying to simulate a 1 ps long plane wave propagating in a dispersive material, with the wave optics transient module. According to the drude-lorentz model, when the frequency of...
29 June 2024 486 1 View
Hi all, I have a several gallons of water mixed with low percent SLS, how can i remove the SLS in a cost-effective manner.
24 June 2024 6,725 3 View
I know that we can categorize a specific linguistic change as either gramaticalization or subjectification (or both involved), but can we also say that specific linguistic change is motivated by...
31 May 2024 2,106 2 View
Hi, I am currently looking at a study where they used a 3 x 2 factorial design where they had 3 diets (A, B, C) and 2 enzyme supplementation levels (0 vs 1 %). Thus giving 6 treatments. Authors...
26 May 2024 4,626 1 View
Hi, I am currently creating a meta-analysis on the effect of growth performance of pigs in response to dietary fiber content in experimental diets as well as the influence of a fiber enzyme...
25 May 2024 8,855 1 View
Examination of sexual assault case and giving evidence in legal situations
14 May 2024 5,228 3 View
This question seeks to identify the most critical hazards on construction sites in Ghana that a mobile app can effectively monitor and report on to improve safety outcomes. The goal is to...
09 May 2024 5,316 1 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
What should be the zeta potential of bovine milk exosomes
25 July 2024 7,376 5 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...
15 July 2024 1,929 2 View
Hello everyone. I am working on my LV concentration porject, I use 500Kda hollowfiber to do it. The HCP and BSA removal rate is nearly 80%, but last time, I failed to run it. The HCP hasn't been...
11 July 2024 5,380 0 View
Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...
11 July 2024 8,484 0 View
I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...
07 July 2024 407 0 View
Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...
07 July 2024 7,967 3 View
I have seen some papers that differentiate C2C12 myoblasts into myotubes by changing the growth medium that contains 10%FBS by a medium containing 2%Horse serum/calf serum + 1% NEAA. What's the...
04 July 2024 4,732 1 View