Estimate Beta is .00, yet it's significant?

Hi everyone, I've run an analysis of a latent 3-way interaction in Mplus (Y on XWZ). The estimate for this path is 0.000. S.E. is 0.000. Est./S.E. is 2.034. Two tailed p-value is 0.042. So,...

08 December 2020 2,132 4 View

What is the double layer capacitance of a 1 sq-cm smooth gold electrode in 0.1N (0.05M) Sulfuric Acid?

Is there an empirical or theoretical standard value for this (at room temperature) and for different electrolyte concentrations? Is there a resource available that provides a chart of values for...

29 November 2020 6,937 2 View

How does the concentration of atmospheric oxygen near ground level vary with time?

Hello, I am interested in how the concentration of O2 varies over time close to ground level. I have been informed that the concentration can drop when an inversion layer is present in the local...

19 November 2020 2,328 3 View

How does one calculate the best volume of nickel gel to use in a protein purification?

We know the capacity of the gel and the total amount of protein in the bacterial lysate from a Bradford assay. What we do not know is what fraction of the total protein is the his-tagged protein...

15 November 2020 4,664 2 View

Final temperature of water leaving an ice bath through copper tubing?

I have water flowing at 4 GPM at 2.5 feet per second through 50ft of 0.375 in. outer diameter tubing with 0.014 in. wall thickness. The water flows through copper tubing into the 50ft coil...

01 November 2020 8,039 5 View

Is it acceptable to publish data that were digitized from a previously published plot/figure (referencing the original figure of course)?

There are many ways to extract original source data from figures and plots in publications. If one were to do that and reanalyze the data in the same or a different way, is that acceptable in a...

28 October 2020 3,822 2 View

Methods to attach glutathione agarose resin to glass slide?

Hello, I am looking to capture a GST tagged protein on a glass slide. I was wondering if anyone had any experiences with glutathoine agarose resin. I realize this is commonly used in affinity...

13 July 2020 1,773 3 View

Where to find predator:prey mass data (for terrestrial carnivores)?

I'm looking for datasets (or databases) in which the body mass of predator and prey are presented. I'm specifically interested in terrestrial predators (snakes, mammals, birds). An example of is...

16 June 2020 2,453 9 View

How to calculate the overlap between animal distributions (Shape or .shp file)?

I would like to calculate the overlap between the distributions of two sets of data (predator & prey). The prey set has got about 250 species, the predator set approximately 5000. For each...

21 May 2020 6,726 3 View

Who can assist with an ingredient design of a Covid 19 Sars-2 Cov protein subunit and adjuvant vaccine?

18 May 2020 1,675 13 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

Questio regarding micro/nano particles fabrication ?

I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...

20 February 2021 199 2 View

Hi..how to isolate crude HDL (all subfractions) from human serum by size exclusion chromatography?

I would like to know about isolation of crude HDL from serum by SEC. Is it possible to perform with a serum sample volume of

19 February 2021 5,576 2 View