Our in-house mycoplasma testing flagged both my media types as positive for mycoplasma, but the cell line media samples I provided all tested negative. The tests were performed on different days and are going to be repeated, but I was wondering if anyone had any insight as to why this might have happened? Could it be a problem with the FBS source for each media type, or are the cells likely to be a false negative? Thanks!
Chandra Somasundaram
You probably have added P/S and fungizone (amphotericin B)to your cell culture which might have got rid of Mycoplasma in your cell line media.
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Malcolm Nobre
It is always better to repeat the test and confirm. How is mycoplasma testing done in-house?
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