My media has tested positive for mycoplasma but the cells being cultured in it didn't. Why?

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Chandra Somasundaram

You probably have added P/S and fungizone (amphotericin B)to your cell culture which might have got rid of Mycoplasma in your cell line media.

Jo Brown

Hi Chandra Somasundaram , thank you for your reply. I did not have any antibiotics or antifungal agents in the media. The media I submitted for testing was the same batch I'd been using to culture the cells.