What are technical problems working with HCT116 colon cancer cell line ? how is it maintained and cultured to study the anticancer effects of a drug?

Technical problems working with HCT116 compared with other cell line Caco-2 and HT29

07 January 2021 2,067 3 View

How to cite a reference for a literature review?

Someone confuse me, he said that when you have some information you want to use it from reference say X, and the reference X inspired this information from reference say Y, then so, I should cite...

21 September 2020 8,630 3 View

What kind of contamination can this be?

01 July 2020 5,790 2 View

How is the coronavirus affecting your research and teaching?

The coronavirus is affecting our lives in many ways, including conducting field trips and laboratory experiments in research and teaching. In-person classes are now being moved to online, which is...

16 March 2020 9,288 6 View

How to choose the right distance method ?

Hi everyone, I have some issues to determine how to pick correctly the right distance calculation method in R. I have two datasets : one containing habitat variables (worldclim data, so...

15 October 2019 7,562 5 View

How can I extract the intensity of electric field maps (E^2 or H^2) in the CST Microwave Studio?

We are looking for a method to extract the power of electric field from the CST Microwave studio simulation. Thanks

14 October 2019 5,769 1 View

How can I determine a metastable phase?

Dear colleagues, Recently, I found a new metastable phase which has not been reported so far. The approximate phase constitution for this metastable phase has been measured by the EDS technique....

17 March 2019 9,725 3 View

In gromacs MD simulation , I run my file with nsteps = 10000000; 2*10000000=20000ps (20ns). Is this correct for 20ns or it should be 10ns ????

I wants to know what's the relationship between nsteps ,ps,ns .How can I calculated exact time for simulation.?????

07 February 2019 8,118 5 View

How can I dry glycerol on nitrocellulose membrane without damaging antibody?

20 April 2017 793 1 View

High fidelity PCR : experimental problem and enzyme choice ?

15 December 2016 5,142 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View