My antibody contains 0.01% sodium azide. Would it be acceptable if I biotinylated the antibody with this concentration of sodium azide?

More Lola Y.'s questions See All

While doing Chronoamperometry, when the light is turned off, why the current is not decreasing sharply?

Here, I have used Ag/AgCl as reference electrode and 0.5M KOH.

29 July 2024 4,538 3 View

Regarding green consumer behaviour ?

HOW TO WRITE GREEN CONSUMER BEHAVIOUR AS A LITERATURE REVIEW

28 June 2024 2,661 0 View

How to have curriculum co-creation in higher education?

In Iran, there is informal co-creation of curriculum in higher education; But formally, no What do we need to implement curriculum co-creation in higher education?

28 May 2024 3,019 14 View

High charging capacity in Na-S battery?

I am working on room temperature sodium sulfur batteries with carbon-based cathode. I have tried many carbonaceous materials like microporous carbon, cnt etc which were reported in literature. But...

10 January 2024 7,505 4 View

Operational excellence and Organizational performance research. What is the approach for PhD study?

I am planning to pursue my Ph.D. study in Healthcare management and want to focus during my study on Operational excellence and Organizational performance in Hospitals. Do you have any...

26 December 2023 2,162 2 View

What is the method of preparation of phosphate buffer at pH 4.7 for alkaloid determination ?

I am working on quantifying alkaloids in a plant extract. I want to prepare a phosphate buffer solution with sodium phosphate dibasic dihydrate with citric acid at pH 4.7. However, sodium...

07 December 2023 6,619 3 View

Isolation of a photo protected PEG compound from diethyl ether?

I Synthesized Nvoc-PEG-NH2 , each time I try to isolate the compound from diethyl ether via vacuum filtration it absorbs moisture and starts to turn to a liquid and I end up loosing most of my...

23 October 2023 7,623 0 View

Are there any methods available for quantitative determination of terpenoids, saponins, glycosides and steroids via UV visible spectrophotometry?

Hi all!! I am working on determining phytochemical constituents in a plant extract. I could not find a reliable protocol for quantitative analysis of terpenoids, saponins, glycosides and steroids...

07 September 2023 5,135 0 View

Would it be possible to know how TLC can be performed for plant based proteins and carbohydrates?

Hi all! I am going to work with plant based proteins and carbohydrates. I want to conduct thin layer chromatography experiments for assessing the presence of proteins and carbohydrates (not in...

24 August 2023 5,918 2 View

Is it necessary to freshly prepare the Brewbacker and Kwack medium ?

I am currently working on pollen studies. I have to use Brewbacker and Quack medium as the pollen germination medium. Is it necessary to prepare it freshly? Thank you very much for your help

18 May 2023 5,920 0 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

Sodium azide in immunohistochemistry?

Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...

23 July 2024 6,314 3 View

How do I avoid HEK293 cell lifting during biotinylation?

I’m doing cell-surface biotinylation with HEK293 cells and after all the washing and biotin incubation steps (washing with PBS ph8, neutralizing unreacted biotin with 40mM Tris ph8) many of my...

23 July 2024 4,740 3 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

Questions of antibodies selection?

Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...

15 July 2024 6,008 5 View

Syed A Ali

Why to take risk; simply dialyze your antibody to get rid of sodium azide and proceed for biotinylation.

Lola Y.

I only have 25-40 microliters of my antibody, so I believe that dialysis would not work.

Well, what is the concentration of antibody? Even after biotinylation, you got to dialyze to remove free biotin.