Lola Y.

10 Questions 10 Answers 0 Followers

Questions related from Lola Y.

How can I dry glycerol on nitrocellulose membrane without damaging antibody?

Hi, I am pipetting an antibody with 50% w/v glycerol. However, I need the antibody solution to dry on the nitrocellulose membrane. How can I do this without damaging the antibody? Thank you.

21 April 2017 946 1 View

When should I block my nc membrane with BSA?

I am creating a sample test of a LFA on a nitrocellulose membrane. I have seen some contradicting protocols regarding blocking the membrane so far. Some suggest doing it before I spot the membrane...

17 April 2016 5,564 2 View

At what setting should I sonicate my carbon nanoparticles?

I will be using the M220 or E210 Covaris sonicator. Any insight would be greatly appreciated! I will be sonicating 1 mg of carbon nanoparticles in 0.1 mL of water and then later with added borate...

15 April 2016 352 3 View

What setting should I use for covaris sonicator?

I am supposed to sonicate 1 mg of carbon nanoparticles in 0.1 mL of MiliQ water using output control 3~27 W, 20 KHz for 5 min. I am also supposed to sonicate three samples of 130 microliters and...

14 April 2016 5,187 2 View

How should I dilute my antigen and antibodies for my test and control line on LFA?

I need to pipet a low molecular weight antigen (around 20 kDA) on my test line and a secondary antibody of anti-rabbit goat antibody- IgG (around 120-160 kDA) on my control line. The...

13 April 2016 8,893 3 View

How do I create Washing Buffer (5 mM BB, 1% (w/v) BSA and Storage Buffer (Sb, 100 mM BB, 1% (w/v))?

I already have the 5 mM Borate Buffer and the 100 mM Borate Buffer. I also have 10% BSA. How do I create 5 mL of the WB and 5 mL of the SB? I need these two buffers to couple carbon nanoparticles...

13 April 2016 1,775 3 View

What is the lowest volume required for Vernier UV-VIS Spectrometer?

I need to confirm that my carbon nanoparticles have been bound to my antibody. I am using the Ocean Optics Red Tide UV-Vis Spectrometer by Vernier. What is the lowest volume required for this to...

13 April 2016 7,310 1 View

How do you block a membrane with BSA?

I am developing a Lateral Flow Assay and need to block a nitrocellulose membrane with BSA for my initial sample test. How do I appropriately coat my membrane with BSA to avoid non-specific...

13 April 2016 2,238 5 View

Is it possible to conjugate antibodies to Acid Black 48 Dye?

I know that because Acid Black 48 is cationic and the antibody is anionic, those two could non-covalently bind. Would this hypothetically work by allowing them to bind through mixing at 4 degrees...

11 April 2016 9,069 2 View

My antibody contains 0.01% sodium azide. Would it be acceptable if I biotinylated the antibody with this concentration of sodium azide?

Has anyone tried to biotinylate an antibody with a similar concentration of sodium azide? I only have 25-40 microliters of my antibody, so I believe that dialysis would not work in this case.

11 April 2016 9,162 3 View