I have synthesized many peptides 6-73amino acid sequences, after purification, some peptides get denatured from pure white powder form, the powder disappeared and just very less oily nature appeared whats the reason for this?
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R.Selva......
The dry peptides may be absorbing moisture from the air and deliquescing. Or, particularly if they are not salts, they may be be amorphous solids or oils at the storage temperature.
Hi Selva! This sounds like the peptide absorbed moisture...this happened to me once with my proteins. You might try to lyophilized extensively before kept at -20 °C. Hope this help!
Dear selva,
1.Due to absorption of moisture peptide get denatured, so you just lyophilize it and then keep store.,
2. Your peptide have some little of reagents like scavanger means get denature, so dissolve it in water and lyophilize it.,
3.If peptide in salt form storage means you won't get this problem.,
Peptide by absorbing moisture this problem, you no need to worry just dissolve that oily denatured form in water and lyophilise it means it will reappear without any change or loss.,
Good Luck..
Only due to moisture from environment, if you are store this under moisture control means this problem won't come.
lyophilization is better option for this problem
For peptides proteins not to degrade, you should prevent any oxidation or water access. To store a cysteine-containing peptide at 4 or minus 20 is not sufficient, you can read off the mass spec how quickly the cysteine and other stuff becomes oxidized. These little household machines, with which you can create a vacuum and then seal, are quite effective, however.
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