R. Selvam

6 Questions 118 Answers 0 Followers

Questions related from R. Selvam

How can I calculate theoretical peptide yield on SPPS? Any Formula?

Any estimation formula for calculating peptide yield before starting synthesis? if there give me a formula with explanation. For example. Resin loading capacity is 0.71mmol /SPPSfor achieving...

02 February 2015 1,353 8 View

During Solid Phase Peptide Synthesis, If we raise temperature up to 35 D C on coupling time does that mean any problem?

Insted of room temperature we raise up to 350C during coupling process means our peptide or system will affect? What will happen?

01 January 2015 479 7 View

How much maximum amino acid can we couple without using any aggregation technique in Solid Phase Peptide Synthesis?

In Solid Phase Peptide Synthesis, maximum how much length are we able to perform? Somebody have did up to 32 residue without using any special technique. Can anyone make this clear to me?

12 December 2014 6,649 4 View

Can any one suggest me the perfect storage condition&Method of Synthetic peptides?

I Need to store synthetic peptide for years, whats the condition and storage method for this, some peptide degeneration takes place even in moisture free -200c. can you suggest any effective...

12 December 2014 6,330 12 View

In HPLC Analysis We got report in Peak Table : Area, Height, Area% & Peack Height% From this Each info What We Exactly Understand??

From this peak table how we know our sample purity? Am analyzing Synthetic peptides and proteins. After HPLC we are doing MALDI,ESI-MS etc but from HPLC what what info we can able to find, please...

12 December 2014 530 7 View

Most of the synthetic peptides/proteins after purification, geting denatured within 60hrs? whats the reason? possible to recover?

I have synthesized many peptides 6-73amino acid sequences, after purification, some peptides get denatured from pure white powder form, the powder disappeared and just very less oily nature...

12 December 2014 9,237 5 View