Insted of room temperature we raise up to 350C during coupling process means our peptide or system will affect? What will happen?
Please read the following
http://www.cem.de/documents/publikationen/spps/dibo_spps.pdf
http://onlinelibrary.wiley.com/doi/10.1002/psc.771/abstract;jsessionid=6A0B801D51D52D1CEB1DF7E81686B431.f04t02?deniedAccessCustomisedMessage=&userIsAuthenticated=false
You should not have any problem by heating your coupling reaction to 35°C. Go for it.
I agree with the answer above. I frequently perform coupling at even higher temperatures without any problems. However, I would not recommend performing Fmoc deprotection at elevated temperatures. Good luck!
Hi, my experience showed that the nature of the coupling agent is relevant. You may need to be careful with EDC as it may give bad surprises sometimes. For other agents, temperature was not an issue at all. Good luck!
If you are increasing temperature means, it won't affect, but i don't know why you are going to increase temperature, i feel by increasing temperature no improvement in coupling yield so no need for this know.,
Any particular reason for this means can you please explain.,
Can you any one say me whats the effect of temperature during the coupling reaction? any use for this? then why need to increase temperature? anybody get more coupling yield when increasing temperature?
Higher temperatures carry the possibility of promoting unwanted side reactions, including epimerisation, aspartimide formation, diketopiperazine formation, and a number of side products depending on the coupling agent used.
Many of these problems are sequence dependent.
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