In Solid Phase Peptide Synthesis, maximum how much length are we able to perform? Somebody have did up to 32 residue without using any special technique. Can anyone make this clear to me?
It depends a lot on the sequence you want to make and its aggregation preferences and having particularly troublesome couplings. We have been able to obtain a fairly pure 52-mer with regular Fmoc/tBu chemistry in an automated synthesizer. You probably want to use some tricks, such as selecting a relatively low resin load (~0.2 mmol/g) and probably make double couplings & capping. By the way, PAL-PEG resins have always been great in our hands.
As told by Eugenio Vazquez, it require inital optimization of conditions and depends many factors. Low loading, swelling of resin in the solvent used (preswelling of resin before coupling is important), efficiency of coupling (you may need multiple coupling as the chain length increases) and choice of resin. Once you optimize the conditions the synthesis generally gives good results (I never did very long sequence of amino acids so I can not specify a number from my experience).
Dear selva, up to 10amino acid can possible without any problem, after 10 means main problem is less solvation due to aggregation, but if you start with below 0.15mmol loading means you can able to couple much up to 62 also reported, but very less yield, and each coupling repeating like this all factor you need to concern., NMP solvent is very best for all coupling.,
Wish You Happy New Year 2015
I Have experience up to 29residue, my senior have up to 47residue, but very poor yield, but if we use some aggregation remote technique means, how much you like that much possible, 249residue also many scientist reported., directly means possible but need to work more, lot of repeating coupling and washing etc. so chemical waste.,
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