How can I calculate theoretical peptide yield on SPPS? Any Formula?

Any estimation formula for calculating peptide yield before starting synthesis? if there give me a formula with explanation. For example. Resin loading capacity is 0.71mmol /SPPSfor achieving...

01 February 2015 1,061 8 View

During Solid Phase Peptide Synthesis, If we raise temperature up to 35 D C on coupling time does that mean any problem?

Insted of room temperature we raise up to 350C during coupling process means our peptide or system will affect? What will happen?

31 December 2014 417 7 View

How much maximum amino acid can we couple without using any aggregation technique in Solid Phase Peptide Synthesis?

In Solid Phase Peptide Synthesis, maximum how much length are we able to perform? Somebody have did up to 32 residue without using any special technique. Can anyone make this clear to me?

11 December 2014 6,587 4 View

In HPLC Analysis We got report in Peak Table : Area, Height, Area% & Peack Height% From this Each info What We Exactly Understand??

From this peak table how we know our sample purity? Am analyzing Synthetic peptides and proteins. After HPLC we are doing MALDI,ESI-MS etc but from HPLC what what info we can able to find, please...

11 December 2014 429 7 View

Most of the synthetic peptides/proteins after purification, geting denatured within 60hrs? whats the reason? possible to recover?

11 December 2014 9,182 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View