Yiqian Zhang

2 Questions 1 Answers 0 Followers

Questions related from Yiqian Zhang

Nested PCR vs. 2nd PCR with same primers?

Hi. I am planning a PCR reaction to amplify a ~500bp region from genomic DNA for T7 endonuclease 1 assay. Some protocols recommend to do a nested PCR, in which you use one set of primers...

10 October 2017 1,869 4 View

Mold contamination in my cell culture?

Hi, I have found something from the liquid nitrogen stock of a 293-derived cell line. Since the cell line is not replaceable, I am hoping to decontaminate my culture. It looks like mold to me, but...

06 June 2017 9,088 2 View