Having the moderator continuous or dichotomous? Using MANOVA or the Process macro (Hayes)?

Hi community! Currently finalising my Master thesis research. I manipulated my independent variable to have three conditions. Originally, I proposed a 3(3 conditions of independent v)x2...

30 January 2021 7,821 6 View

After data collection we saw that items were missing in survey, is there any way to fix this?

Unfortunately, we just realized after data collection, when we wanted to start analyzing our data, that some items were missing in the online survey. The survey consisted of three parts (three...

21 April 2020 6,736 11 View

Can you grow MDA-MB-231 as spheroids in ULA plates without Matrigel or any other ECM?

Can MDA-MB-231 cells be grown as spheroids in just ultra low attachment plates, or do they require some extracellular matrix to give them structure?

10 April 2019 5,172 3 View

To purify DNA bound to antibody (ChIP-seq) I currently use Phenol-chloroform. Can I incubate over a weekend with 100% ethanol instead of overnight?

If you require further details before answering please let me know, thanks

25 July 2017 8,404 2 View

What could be the reason for negative OCR values in Seahorse Oxygraph?

I have started working with the Seahorse XF96 from Agilent starting with the recommended New Cell Line Characterization protocol to determine cell density and FCCP concentration. I have some...

18 April 2017 360 3 View

What are sound self-report instruments to assess attachment style?

A German language version of the instrument should have been established and validated, as we plan to use it in a validation study of another instrument. The measure should be suitable for the use...

15 August 2016 3,291 2 View

Which correction for type 1 error due to multiple correlations?

I'm trying to find a test to correct for type 1 error due to multiple correlations (15 variables). Although Bonferroni is generally considered too conservative, I'm having trouble finding any...

21 February 2016 1,463 10 View

Can one fit the Multidimensional Random Coefficients Multinomial Logit Model in SPSS or R?

I would like to fit a multidimensional Rasch model to a binary questionnaire data using SPSS or R. The data are multidimensional as the constructs measured use parallel design and common stimulae...

05 June 2014 1,101 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

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03 March 2021 5,360 2 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View