Is there a TCR transgenic mouse model on FVB background that produce CD8+ T cells specific to e.g. ovalbumin or hemagglutinin?

07 October 2021 0 648 Report

I am planning an in vitro mouse tumor cell/T cell co-culture assay. Tumor cells are derived from a GEMM that was generated in FVB mice. I am now searching for an transgenic TCR FVB mouse model that produce CD8+ T cells specific to an antigen like ovalbumin or hemagglutinin. There are comparable models with ovalbum-specificity (OT-I mice in C57BL/6 strain) and hemagglutinin-specificity (Clone 4 mice in BALB/c strain). Can anyone recommend a suitable transgenic TCR FVB model?

Badges
Science topic

Similar topics
Eutheria
Rodentia
Muridae
Murinae
Mice

More Leonie Ratz's questions See All

To which Reichsgruppe did coalmining belong?

During my research for my bachelors thesis into the classification of mining in the Reichsgruppe under the Nazi regime, I came across two different classifications: On the one hand, Boris Gehlen...

06 March 2024 3,442 3 View

In the Fritz and MacKinnon (2007) paper, which type 1 error is used as the basis for the power analysis for a mediation with bootstrapping?

Dear all, As part of my bachelor thesis, I am using a simple mediation with bootstrapping procedure. I would now like to calculate the power of my analysis. For guidance, I have read the 2007...

29 November 2023 7,834 2 View

How to conduct a spearmans rank correlation on SPSS?

Hi, I'm currently analysing data for my quantitative dissertation and I'm struggling with how to do it. My data has come from 3 questionnaires with 148 questions in total and it is at an ordinal...

24 April 2022 3,326 3 View

Imputation in the (near) absence of meaningful correlation?

I have a dataset with some data missing completely at random (most < 10% missing). So far, so good. I am planning on computing regression models. However, the variables in my regression model,...

28 October 2021 6,459 4 View

Having the moderator continuous or dichotomous? Using MANOVA or the Process macro (Hayes)?

Hi community! Currently finalising my Master thesis research. I manipulated my independent variable to have three conditions. Originally, I proposed a 3(3 conditions of independent v)x2...

30 January 2021 8,095 6 View

Can I use a moderation hypothesis if it can't be justified with previous research?

Hello everyone, I am writing the literature review for my masters thesis. I use a moderated mediation but I cannot fully justify the moderation with the existing literature. Can I still use this...

22 April 2020 6,086 3 View

After data collection we saw that items were missing in survey, is there any way to fix this?

Unfortunately, we just realized after data collection, when we wanted to start analyzing our data, that some items were missing in the online survey. The survey consisted of three parts (three...

21 April 2020 6,960 11 View

How can I implement a quasi static analysis in ANSYS Workbench?

I want to simulate the deformation and the plastic strains of a slope with non linear material behaviour (I used the Mohr-Coulomb Model) which experiences a load of 0,6*g. I want ANSYS to consider...

15 April 2020 2,679 0 View

Can you grow MDA-MB-231 as spheroids in ULA plates without Matrigel or any other ECM?

Can MDA-MB-231 cells be grown as spheroids in just ultra low attachment plates, or do they require some extracellular matrix to give them structure?

10 April 2019 5,387 3 View

Is there research on the variation of German adnominal possessive constructions?

I am searching for research on the variation of German adnominal possessive constructions (s-construction: Peter-s Jacke 'peter's jacket' vs. of/von-construction die jacke von Peter 'the jacket of...

12 February 2019 7,568 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View