Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View

How to estimate 2SLS with different instruments for different endogenous variables?

To be specific, I want to conduct an IV regression in stata with the following three equations. EQ1: Y1 ~Y2 Y3 X EQ2: Y2 ~ Z1 EQ3: Y3 ~ Z2 Both Y2 and Y3 are endogenous. However, Z1 and Z2 are...

23 February 2021 6,266 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

Sample modeling in ABAQUS software and its relationship with the real world?

About crack modeling in ABAQUS: Mode I (pure-tensile cracking) and Mode II (pure-shear cracking), these assumptions may be accruing in theoretical approach (ABAQUS) not practical in Lab....

19 February 2021 6,156 1 View

Making my research papers available for readers?

17 February 2021 8,533 2 View

How does a freeze dryer destruct your solid sample?

Several times I lost my sample during freeze drying. There was no solid or powder in the vial after freeze drying. I'm sure sample contained solute because i took its NMR spectrum. I covered the...

15 February 2021 6,749 2 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

How to measure/estimate media pH inside the incubator?

I am having an issue with measuring the pH of my media. I use D-MEM/F12 media (without buffer) and try to adjust it to pH = 7.4. Since I incubate it @ 37C / 5% CO2, I added 2.5 g/L of NaHCO3...

18 January 2021 3,816 1 View

Just did Fluid Flow and Heat Transfer in a Mixing Elbow using ANSYS Fluent as per instructed but I got my net result in negative in Flux Reports. why?

Got negative results in flux reports can I get some solid answers for it, so I can reconsider on my problem, briefly can anyone describe briefly the issue that may have caused such answers? As...

08 January 2021 880 3 View

Can two seperate variables, one a strong negative correlation and the other moderate negative result into a strong positive in a multiple regression?

I selected two individual variables with very decent negative correlation coefficient to fit in the multiple regression analysis. I ran it, whilst the regression coefficients remained negative,...

03 January 2021 3,432 10 View

Is it always mandatory to report and test the validity and reliability of a newly developed questionnaire?

Hello everyone! Currently, I am doing an observational, cross-sectional study on knowledge, atittude, and practice towards thalassemia in Indonesia. My area of interest is in population studies....

16 December 2020 3,725 16 View

Lipid structures disappear upon negative stainging. Does anyone knows this effect?

13 December 2020 1,559 3 View

How can I get better picture for the attached TEM Image??

I used TEM equipped with tungsten gun to obtain attached image (several other too, like shown). Side blotting technique followed by negative staining (1% PTA for 30 sec) was used. I cannot get the...

31 October 2020 1,902 5 View

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29 October 2020 3,665 6 View

DNA extraction from bacterial culture?

Is it an effective method to isolate DNA from Gram-negative bacteria with only the use of a boil and freeze-thaw protocol, without any chemical purification? Also, is there a media to maintain the...

06 October 2020 6,890 8 View

How many days later the Covid-19 patient will be in negative result from his/her initial day of infection?

The opinions are different. But actually, it is essential to know how long time the Covid-19 patient will be in negative result from his/her initial day of infection.

30 September 2020 6,505 19 View