I'm just learning the ePrime 3.0 software and have created a test. How can I put this test on a tablet for subjects to use for data collection?

This is probably a really basic question, but I can't figure it out. I've loaded the test on a USB and transferred to the tablet, but am unable to get it to run. Any help would be wonderful. Thank...

23 December 2020 5,202 3 View

How do I calculate transition probabilities for a sequence of syllables in a set of bird songs using R?

I would like to automatically generate a Markov chain analysis for my set of bird songs that I have assigned syllable categories to using R. I have around 70 songs (each from an individual bird)...

13 October 2020 4,833 2 View

Someone knows of a DNA purification protocol from polysaccharides (for brown seaweed extracts)?

Hi, I have this "bad" DNA extract of a Fucus spp. and I want to purifying this because the PCR analysis that I need don't show results. I alredy try to extract twice the DNA both with the Genomic...

06 October 2020 1,500 1 View

Inconsistent protein expression for different maxipreps?

I have been having large variability in protein expression from transfections of mammalian cells with DNA from different maxipreps, even though the plasmid is the same. I have been using the...

02 October 2020 2,924 3 View

How to prepare a vial of Sigma Adjuvant System (Ribi) when needing multiple immunogens/at different immunisation points?

https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Datasheet/5/s6322dat.pdf Has anyone prepared a vial of sigma adjuvant systems (Ribi's) when they either aren't using the whole...

02 September 2020 7,451 2 View

Who is investigating artificial intelligence / machine learning for leadership augmentation and resource distribution?

I've been searching various A.I. projects and have found very little on the role A.I. can play in leadership and government. If we imagine a leader who cares about their constituency and does not...

09 August 2020 1,213 2 View

How do I assess when to use "match between runs" for LFQ analysis on Maxquant?

Hi Proteomics enthusiasts, I am currently analyzing DNA pulldown data (treated vs non-treated) using Label-free quantification on Maxquant. I am trying to assess if a protein is present in one...

04 August 2020 1,090 6 View

Could you recommend a scale that measures group intelligence or the predisposition to work in a group?

For my thesis project, based on the work of Reeves and Nass reported in the book "The Media Equation", I would need a scale that goes to measure group intelligence or the predisposition to work in...

25 July 2020 1,294 3 View

Have anyone ever done a mutagenesis of DNA with a CAG promoter?

I need to do a site-directed mutagenesis of a DNA insert in a pCAX plasmid with a CAG promoter. I have used "QuickChange II XL Site-Directed Mutagenesis Kit" (Agilent Technologies) to make a...

21 July 2020 4,618 1 View

What is the minimum baseline data set for TAU-U analysis in single case research?

Hello, I'm looking at calculating the TAU-U effect size for my single case research data set. I'm struggling to find any literature on the minimum number of baseline data points to compute TAU-U,...

21 June 2020 445 1 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View