Alaleh Rezalotfi

5 Questions 15 Answers 0 Followers

Questions related from Alaleh Rezalotfi

Does anybody know a good tool for analysis of gene editing?

Hi, I'm working with CRISPR Knock-in experiments, and I need a tool to check the insertion I have. I've been using ICE from Synthego for analysis of DNA sequencing data. However ICE is not able...

28 July 2021 4,174 5 View

In vitro T cell response to virus antigenes?

I would like to design an in vitro assay in which I examine the reaction of T cells among PBMCs to the COVID-19. Due to safety issues, I can't directly culture the virus with PBMCs. On the other...

03 June 2020 9,096 8 View

How much protein (microgram) can be extracted from 15 ml of a cancer cells line conditioned medium?

I'm going to extract total protein from 15 ml of a cancer cell line 24-hour conditioned medium. I need to know how much protein I can extract from this amount of CM, and is this enough for mass...

26 December 2018 3,478 7 View

How to convert international unit per ml (IU/ml) into Units per ml (U/ml) ?

I could not find a source to show the difference between the two. but I am wondering if they are the same. I would be thankful if anyone can help me.

12 November 2017 1,088 5 View

Can both surface and intracellular staining be done in the intracellular staining process?

Is it possible to stain cells with both surface and intracellular antibodies in one step after fixation and permeabilization ? I think it makes the staining procedure shorter and may improve cell...

24 August 2017 5,823 10 View