5 Questions 16 Answers 0 Followers
Questions related from Alaleh Rezalotfi
Hi, I'm working with CRISPR Knock-in experiments, and I need a tool to check the insertion I have. I've been using ICE from Synthego for analysis of DNA sequencing data. However ICE is not able...
28 July 2021 4,185 5 View
I would like to design an in vitro assay in which I examine the reaction of T cells among PBMCs to the COVID-19. Due to safety issues, I can't directly culture the virus with PBMCs. On the other...
03 June 2020 9,105 8 View
I'm going to extract total protein from 15 ml of a cancer cell line 24-hour conditioned medium. I need to know how much protein I can extract from this amount of CM, and is this enough for mass...
26 December 2018 3,492 7 View
I could not find a source to show the difference between the two. but I am wondering if they are the same. I would be thankful if anyone can help me.
12 November 2017 1,097 5 View
Is it possible to stain cells with both surface and intracellular antibodies in one step after fixation and permeabilization ? I think it makes the staining procedure shorter and may improve cell...
24 August 2017 5,885 10 View