Can I prepare MitoTracker aliquots, by diluting it in cell culture medium at a certain concentration, and then store them? If yes, is it better to store the aliquots at -20 or -80 °C? How long they can be stored?
Prepare a stock solution in DMSO. This stock solution can be stored at ‒20 ºC for at least 10-12 months or the duration of storage could also be mentioned in the product datasheet. Avoid multiple freeze-thaw cycles. You can make aliquots of the stock solution. To prepare working solution dilute the stock in culture media on the day of experiment. Do not prepare aliquots in culture medium and store them.
Thank you Malcolm Nobre. My question was exactly about the working solution, not the stock solution. So, according to your experience, it's better to prepare the WS on the day of the experiment.