Alessandra Maria Vitale

6 Questions 7 Answers 0 Followers

Questions related from Alessandra Maria Vitale

Whole spheroids immunofluorescence staining protocol?

Hi everyone! I want to perform an immunofluorescent staining of spheroids from human glioblastoma cells fixed in 4% PFA. I found many protocols and papers suggesting different solutions and times...

03 March 2024 4,618 2 View

How to analyze mitochondria morphology (shape), and measure mitochondria length and number in MitoTracker-stained cells?

I would like to analyze mitochondria morphology (shape), and measure mitochondria length and number in Mitotracker-stained HDFs cells (40X and 60X images). Is there an easy-to-use tool or software...

19 March 2022 3,682 4 View

Does the GFP fluorescence disappear in fixed transfected cells?

I transfected my cells (primary human dermal fibroblasts) with a plasmid containing the gene of interest and a C-terminal turboGFP tag. I could see the turboGFP fluorescence using a fluorescence...

12 November 2021 7,273 3 View

MitoTracker aliquots preparation and store?

Can I prepare MitoTracker aliquots, by diluting it in cell culture medium at a certain concentration, and then store them? If yes, is it better to store the aliquots at -20 or -80 °C? How long...

08 November 2021 9,511 3 View

How long does the staining with Mitotracker last after cells fixation?

Hi everyone. I stained my cells with Mitotracker Red CMXRos (Invitrogen, catalog number M7512) 100nM, for 30’, and then I visualized the staining with a fluorescence microscope, washed cells, and...

22 June 2021 3,021 4 View

Could plasmid size affect the efficiency of Lipofectamine 3000-based transfection?

I’m currently trying to transfect LAN5 cells (human neuroblastoma cell line) using the Lipofectamine 3000 transfection reagent, both with the vector pCMV6-AC-GFP expressing the human heat shock...

15 April 2021 8,984 4 View