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Questions related from Alessandra Maria Vitale
Hi everyone, I’m having trouble maintaining the viability of my SVG p12 Fetal Glial Cells in culture. They don’t survive for more than two-three days, and once they reach 30% confluency, they...
30 January 2025 2,668 1 View
Hi everyone! I want to perform an immunofluorescent staining of spheroids from human glioblastoma cells fixed in 4% PFA. I found many protocols and papers suggesting different solutions and times...
03 March 2024 4,660 2 View
I would like to analyze mitochondria morphology (shape), and measure mitochondria length and number in Mitotracker-stained HDFs cells (40X and 60X images). Is there an easy-to-use tool or software...
19 March 2022 3,721 4 View
I transfected my cells (primary human dermal fibroblasts) with a plasmid containing the gene of interest and a C-terminal turboGFP tag. I could see the turboGFP fluorescence using a fluorescence...
12 November 2021 7,315 3 View
Can I prepare MitoTracker aliquots, by diluting it in cell culture medium at a certain concentration, and then store them? If yes, is it better to store the aliquots at -20 or -80 °C? How long...
08 November 2021 9,531 3 View
Hi everyone. I stained my cells with Mitotracker Red CMXRos (Invitrogen, catalog number M7512) 100nM, for 30’, and then I visualized the staining with a fluorescence microscope, washed cells, and...
22 June 2021 3,055 4 View
I’m currently trying to transfect LAN5 cells (human neuroblastoma cell line) using the Lipofectamine 3000 transfection reagent, both with the vector pCMV6-AC-GFP expressing the human heat shock...
15 April 2021 9,011 4 View
