Can someone suggest on what miRNAs can be used as targets to detect healthy serum and plasma samples based on Taqman miRNA assay? I have used the spike in- ath-miR-159a during RNA isolation process and analyzed the concentration of serum and plasma using fragment analyzer. I then went on to perform a qPCR, since my basic goal is to determine whether serum/plasma is a good model to be investigated. I used an endogenous control, mir-423-5p which is proposed to be a good reference gene that can be used for serum and plasma. I got the Ct values and with just one sample analyzed so far, it seems to me like plasma has a better Ct value than serum. I am yet to investigate on few more healthy controls. But, I also wanted to investigate the difference in expression between serum and plasma, for which I am on a lookout for a good target gene that can be detected in both serum and plasma of healthy subjects. Any inputs would be appreciated!
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