How to make quantification of specific bacteria added to the soil?

I'm looking for the best method for quantification of specific bacteria added to the soil. I'm planning an experiment with bacterial addition and would like to check its survival in the soil...

29 September 2020 8,596 3 View

LCA in GaBi. How the credits for avoided processes could be included in the main plan ?

Hello, Could someone confirm whether my approach to include the credits for avoided processes in the LCA GaBi software is a correct one please? Please see the attached jpegs. I have just added...

20 July 2020 8,435 1 View

What internal control for qPCR of fungi detection in plants is recommended ?

I need to perform qPCR detection of specific fungal species in plant (strawberry). I need to include internal control (housekeeping gene) for plant, I have found that some people use COX gene, but...

11 July 2019 7,756 2 View

Are we still people or cogs of the IF machine ?

when publishing many of us have two problems: 1. editors predict IF and the problem of your publication in fact is not bad quality or out of scope 2. unofficially journal publishes articles mainly...

16 April 2018 9,439 3 View

Is there any chance or some kind of RIF "Real Impact Factor" that would measure the outcomes of the published research for the founding community?

@Witold Bogusz asked a question about "the purpose of research and innovation" in various countries as the starting point is not the same worldwide. Problems to be resolved in local context...

01 January 1970 8,951 1 View

What is the role of bearing microparticles of obstetric complications STBM in: preeclampsia, amniotic-fluid embolism, placental abruption?

01 January 1970 2,302 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View