How to make quantification of specific bacteria added to the soil?

I'm looking for the best method for quantification of specific bacteria added to the soil. I'm planning an experiment with bacterial addition and would like to check its survival in the soil...

29 September 2020 8,596 3 View

LCA in GaBi. How the credits for avoided processes could be included in the main plan ?

Hello, Could someone confirm whether my approach to include the credits for avoided processes in the LCA GaBi software is a correct one please? Please see the attached jpegs. I have just added...

20 July 2020 8,435 1 View

What internal control for qPCR of fungi detection in plants is recommended ?

I need to perform qPCR detection of specific fungal species in plant (strawberry). I need to include internal control (housekeeping gene) for plant, I have found that some people use COX gene, but...

11 July 2019 7,756 2 View

Are we still people or cogs of the IF machine ?

when publishing many of us have two problems: 1. editors predict IF and the problem of your publication in fact is not bad quality or out of scope 2. unofficially journal publishes articles mainly...

16 April 2018 9,439 3 View

MinION Oxford Nanopore Technologies in metagenomics

If anyone is familiar with Minion Nanopore Technology in metagenomic studies ? What is your opinion, is it cost-efficient and if this technology improved it's error rate ? Can anyone share...

06 August 2017 5,922 2 View

Is there any chance or some kind of RIF "Real Impact Factor" that would measure the outcomes of the published research for the founding community?

@Witold Bogusz asked a question about "the purpose of research and innovation" in various countries as the starting point is not the same worldwide. Problems to be resolved in local context...

01 January 1970 8,951 1 View

What is the role of bearing microparticles of obstetric complications STBM in: preeclampsia, amniotic-fluid embolism, placental abruption?

01 January 1970 2,302 3 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Plant research: Watering uniformity/consistency. How important is it?

Trying to gather insight from an unresolved discussion with a colleague here, and either the available literature surrounding this topic and/or my keywords in search of are lacking. Context:...

21 February 2021 6,389 3 View

How to dissolve lyophilized silk fibroin?

Dear members of the community, I am trying to concentrate silk fibroin to 25 % (w/v). Nevertheless, i am struggling with the concentration step. I successfully achieve concentrations of 5-6 % of...

16 February 2021 1,808 1 View

How does a freeze dryer destruct your solid sample?

Several times I lost my sample during freeze drying. There was no solid or powder in the vial after freeze drying. I'm sure sample contained solute because i took its NMR spectrum. I covered the...

15 February 2021 6,749 2 View

Can i use the " weight moisture" ( humidite ponderale in French) of plants to describe their efficiency in using water?

Weight moisture is : water content/ dry weight , if for example it is 4 it means that this plant have for each 1gam of dry mass 4 grams of water. can i use this to interpret the plant use of...

14 February 2021 5,784 1 View

How to determine the drug loading and the drug release capacity of a polymer micelle using UV spectroscopy?

Currently, I am working on PLA-PEG copolymer micelle for the drug delivery system. I have seen several papers where some researcher talks to centrifuge the drug-loaded micelle to separate the...

13 February 2021 5,822 7 View