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Questions related from Murugan Nandagopal
Am working on a essential gene for bacterial growth. In silico virtual studies based small molecule has been identified and studied in vitro. We expected, when a protein is inhibited for its...
18 January 2018 9,754 4 View
My purpose is to clone a human target gene (for eg:insulin gene) into a cell line and express the same in cell line for research purpose. My plan is to take RNA from human tissue and convert it to...
24 February 2017 4,394 1 View
Recently I found there are many CRISPR genes among my Pseudomonas aeruginosa genome study. My query is whether these could be adapted from any viral or other microbes or it is innate in some of...
24 August 2016 1,837 5 View
Elastase assay using Elastin-Congo Red assay principle i needed ? Though i understood that Elastin is a substrate for Elastase enzyme, which is bound with the elastin congo red upon incubation...
11 June 2016 6,140 1 View
Recently we tried to standardize Real Time PCR from a clinical samples extracted cDNA or DNA and upon quantification results ends with A260/280=2.0, 2.5, 2.6 and the concentration maximum of...
10 March 2016 1,620 2 View
Can anybody explain: Is the RND efflux pump genes MexAB-oprM present in all the Grm Negatives , but hyperexpressed only in repressor mutant strains? Presence of MexAB-oprM, MexCD-oprJ, MexXY-oprN...
02 March 2016 329 7 View
Dear researchers, I have identified the presence of efflux pump genes in only 65% of Pseudomonas and absent in 35% of Pseudomonas strains. Where detection of the gene by PCR targeting MexAB-oprM...
24 November 2015 7,324 1 View
Hi, I have found a chemical compound has good antibacterial activity. I want to confirm that this commercially available compound has any host cell toxicity (Human) not related to cancer. Since my...
24 July 2015 9,105 19 View
As per my research, drives me to inhibit a particular gene which codes for a protein involves in multiple functions. After treating the cells with my designed drug against that protein, i have...
15 June 2015 7,064 6 View
I have indicated some pathogenic genes of P aeruginosa identified by genome sequencing in a paper, where the reviewer wanted to do a functional study for the genes which I claimed, but I have...
11 February 2015 6,888 7 View
Dear scientist, Am having a small molecule ( selected by in silicomethod) to target a gene or protein (x) which involves in cell division of bacteria. 1) I want to prove that the small molecule...
26 November 2014 4,422 3 View
Dear scientists, Out of my curiosity, when i analysed a patient harbouring pseudomonas strain with higher resistance, i have taken the toilet swab, wash basin swab and air sampling of the patient...
15 September 2014 380 5 View
Dear scientists, Am working in pseudomonas bacteria, where i could find lot of resistant genes, but when i extract the same with plasmid DNA extraction kit, no dna could be extracted. Is there any...
01 September 2014 8,768 8 View
I'm doing NGS and I published a whole genome of Pseudomonas aeruginosa bacteria. I want to construct a phylogeny based on whole genome not with either 16s or housekeeping genes. Is there any...
23 August 2014 2,152 9 View
Dear friends, Other than sequencing error, what could be the reason for this high no of Pseudogenes in many bacteria?
05 August 2014 408 3 View
COVID-19, causes 80% asymptomatic and only 20% critical situation among patients, who are that 20% exactly? As per my understanding, 20% people belongs to following criteria 1. Aged above 60+ 2....
01 January 1970 3,381 4 View
