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Questions related from Murugan Nandagopal
My purpose is to clone a human target gene (for eg:insulin gene) into a cell line and express the same in cell line for research purpose. My plan is to take RNA from human tissue and convert it to...
24 February 2017 4,188 1 View
Recently we tried to standardize Real Time PCR from a clinical samples extracted cDNA or DNA and upon quantification results ends with A260/280=2.0, 2.5, 2.6 and the concentration maximum of...
10 March 2016 1,441 2 View
Dear researchers, I have identified the presence of efflux pump genes in only 65% of Pseudomonas and absent in 35% of Pseudomonas strains. Where detection of the gene by PCR targeting MexAB-oprM...
24 November 2015 7,219 1 View
As per my research, drives me to inhibit a particular gene which codes for a protein involves in multiple functions. After treating the cells with my designed drug against that protein, i have...
15 June 2015 6,881 6 View
Dear scientist, Am having a small molecule ( selected by in silicomethod) to target a gene or protein (x) which involves in cell division of bacteria. 1) I want to prove that the small molecule...
26 November 2014 4,273 3 View
Dear scientists, Am working in pseudomonas bacteria, where i could find lot of resistant genes, but when i extract the same with plasmid DNA extraction kit, no dna could be extracted. Is there any...
01 September 2014 8,602 8 View
I'm doing NGS and I published a whole genome of Pseudomonas aeruginosa bacteria. I want to construct a phylogeny based on whole genome not with either 16s or housekeeping genes. Is there any...
23 August 2014 2,093 9 View
Dear friends, Other than sequencing error, what could be the reason for this high no of Pseudogenes in many bacteria?
05 August 2014 252 3 View
COVID-19, causes 80% asymptomatic and only 20% critical situation among patients, who are that 20% exactly? As per my understanding, 20% people belongs to following criteria 1. Aged above 60+ 2....
01 January 1970 3,217 4 View
