14 Questions 55 Answers 0 Followers
Questions related from Murugan Nandagopal
Am working on a essential gene for bacterial growth. In silico virtual studies based small molecule has been identified and studied in vitro. We expected, when a protein is inhibited for its...
18 January 2018 9,676 4 View
My purpose is to clone a human target gene (for eg:insulin gene) into a cell line and express the same in cell line for research purpose. My plan is to take RNA from human tissue and convert it to...
24 February 2017 4,210 1 View
Elastase assay using Elastin-Congo Red assay principle i needed ? Though i understood that Elastin is a substrate for Elastase enzyme, which is bound with the elastin congo red upon incubation...
11 June 2016 6,071 1 View
Recently we tried to standardize Real Time PCR from a clinical samples extracted cDNA or DNA and upon quantification results ends with A260/280=2.0, 2.5, 2.6 and the concentration maximum of...
10 March 2016 1,455 2 View
Can anybody explain: Is the RND efflux pump genes MexAB-oprM present in all the Grm Negatives , but hyperexpressed only in repressor mutant strains? Presence of MexAB-oprM, MexCD-oprJ, MexXY-oprN...
02 March 2016 246 7 View
Dear researchers, I have identified the presence of efflux pump genes in only 65% of Pseudomonas and absent in 35% of Pseudomonas strains. Where detection of the gene by PCR targeting MexAB-oprM...
24 November 2015 7,234 1 View
As per my research, drives me to inhibit a particular gene which codes for a protein involves in multiple functions. After treating the cells with my designed drug against that protein, i have...
15 June 2015 6,894 6 View
I have indicated some pathogenic genes of P aeruginosa identified by genome sequencing in a paper, where the reviewer wanted to do a functional study for the genes which I claimed, but I have...
11 February 2015 6,841 7 View
Dear scientist, Am having a small molecule ( selected by in silicomethod) to target a gene or protein (x) which involves in cell division of bacteria. 1) I want to prove that the small molecule...
26 November 2014 4,284 3 View
Dear scientists, Out of my curiosity, when i analysed a patient harbouring pseudomonas strain with higher resistance, i have taken the toilet swab, wash basin swab and air sampling of the patient...
15 September 2014 334 5 View
Dear scientists, Am working in pseudomonas bacteria, where i could find lot of resistant genes, but when i extract the same with plasmid DNA extraction kit, no dna could be extracted. Is there any...
01 September 2014 8,613 8 View
I'm doing NGS and I published a whole genome of Pseudomonas aeruginosa bacteria. I want to construct a phylogeny based on whole genome not with either 16s or housekeeping genes. Is there any...
23 August 2014 2,102 9 View
Dear friends, Other than sequencing error, what could be the reason for this high no of Pseudogenes in many bacteria?
05 August 2014 264 3 View
COVID-19, causes 80% asymptomatic and only 20% critical situation among patients, who are that 20% exactly? As per my understanding, 20% people belongs to following criteria 1. Aged above 60+ 2....
01 January 1970 3,232 4 View
