Jarosław Grządziel

7 Questions 16 Answers 0 Followers

Questions related from Jarosław Grządziel

What makes using nanopore sequencing difficult ?

I would like to know what are the main limitations of using nanopore sequencing, what are the most difficult steps ? How about support, applications available (are apps easy to use and free of...

12 March 2021 9,318 2 View

What internal control for qPCR of fungi detection in plants is recommended ?

I need to perform qPCR detection of specific fungal species in plant (strawberry). I need to include internal control (housekeeping gene) for plant, I have found that some people use COX gene, but...

12 July 2019 7,854 2 View

Freeze drying / lyophilization in Eppendorf tubes ? And the most suitable medium for this process ?

I want to ask if anybody tried to perform a freeze drying / lyophilization of bacteria or fungi collection, using Eppendorf tubes (e.g. 5 ml with a screw cap) instead of glass vials with...

18 January 2019 4,068 1 View

MinION Oxford Nanopore Technologies in metagenomics

If anyone is familiar with Minion Nanopore Technology in metagenomic studies ? What is your opinion, is it cost-efficient and if this technology improved it's error rate ? Can anyone share...

07 August 2017 6,006 2 View

DGGE - Why DNA remains in wells ?

Hi, Im trying to begin with DGGE experiments, but lot of DNA remains in wells. Picture in attachment My DGGE condidtions: Size of amplicon: ~ 500 bp (V6/V9 region) Ammount od DNA - 750 ng per...

04 March 2016 227 15 View

Does thrombin require calcium for its activity?

I would like to ask if there is calcium really unnecessary for thrombin activity ? I've found one publication, where they claimed that thrombin is Ca-dependent enzyme ... but there was no...

17 March 2015 5,655 12 View

Nanopore sequencing vs Illumina - which technology is better for a new, unexperienced lab?

I would like to know what are the main limitations of using nanopore sequencing, what are the most difficult steps ? How about support, applications available (are apps easy to use and free of...

01 January 1970 8,574 4 View