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Questions related from Jarosław Grządziel
I would like to know what are the main limitations of using nanopore sequencing, what are the most difficult steps ? How about support, applications available (are apps easy to use and free of...
12 March 2021 9,370 2 View
Hi everyone, I'm looking for a tool that could help to assign automatically microbial attributes (e.g. oxidative/non-oxidative metabolism, G+/G-, human diseases causation, endospores formation...
07 October 2019 3,619 2 View
I need to perform qPCR detection of specific fungal species in plant (strawberry). I need to include internal control (housekeeping gene) for plant, I have found that some people use COX gene, but...
12 July 2019 7,921 2 View
I want to ask if anybody tried to perform a freeze drying / lyophilization of bacteria or fungi collection, using Eppendorf tubes (e.g. 5 ml with a screw cap) instead of glass vials with...
18 January 2019 4,118 1 View
If anyone is familiar with Minion Nanopore Technology in metagenomic studies ? What is your opinion, is it cost-efficient and if this technology improved it's error rate ? Can anyone share...
07 August 2017 6,064 2 View
Hi, Im trying to begin with DGGE experiments, but lot of DNA remains in wells. Picture in attachment My DGGE condidtions: Size of amplicon: ~ 500 bp (V6/V9 region) Ammount od DNA - 750 ng per...
04 March 2016 280 15 View
I would like to ask if there is calcium really unnecessary for thrombin activity ? I've found one publication, where they claimed that thrombin is Ca-dependent enzyme ... but there was no...
17 March 2015 5,703 12 View
01 January 1970 8,626 4 View
