How to make quantification of specific bacteria added to the soil?

I'm looking for the best method for quantification of specific bacteria added to the soil. I'm planning an experiment with bacterial addition and would like to check its survival in the soil...

29 September 2020 8,596 3 View

LCA in GaBi. How the credits for avoided processes could be included in the main plan ?

Hello, Could someone confirm whether my approach to include the credits for avoided processes in the LCA GaBi software is a correct one please? Please see the attached jpegs. I have just added...

20 July 2020 8,435 1 View

Are we still people or cogs of the IF machine ?

when publishing many of us have two problems: 1. editors predict IF and the problem of your publication in fact is not bad quality or out of scope 2. unofficially journal publishes articles mainly...

16 April 2018 9,439 3 View

MinION Oxford Nanopore Technologies in metagenomics

If anyone is familiar with Minion Nanopore Technology in metagenomic studies ? What is your opinion, is it cost-efficient and if this technology improved it's error rate ? Can anyone share...

06 August 2017 5,922 2 View

Is there any chance or some kind of RIF "Real Impact Factor" that would measure the outcomes of the published research for the founding community?

@Witold Bogusz asked a question about "the purpose of research and innovation" in various countries as the starting point is not the same worldwide. Problems to be resolved in local context...

01 January 1970 8,951 1 View

What is the role of bearing microparticles of obstetric complications STBM in: preeclampsia, amniotic-fluid embolism, placental abruption?

01 January 1970 2,302 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View