Many diseases like cancer, Alzheimer's and cardiovascular diseases are directly or indirectly controlled by miRNA. But is it miRNA used in the early detection of these diseases?
http://edrn.nci.nih.gov/
My question is there any microRNA based diagnostic techniques are in use for early detection of disease.
Hope it could be helpful:
http://www.genomeweb.com/rnai/exiqon-plans-launch-two-mirna-diagnostics-2014
and
http://circres.ahajournals.org/content/110/3/496.full
This specific field appears very appealing and promising and your question bvery interesting
Yes, as this field became very fascinating and fast moving. Today most of the diseases are regulated by microRNA. It is necessary to deeply investigate in this field and to develop new diagnostic tools.
Yes this question is intriguing.
It is true that there are aberrant regulations of microRNA networks in multiple different diseases. But diseases are very complicated and if you think that a single microRNA can be responsible for a complex trait in 100% of the cases and 100% of the patients the answer unfortunately is no.
MicroRNAs do not work alone but in network and their activity is regulated by RNA-binding proteins. Moreover the seed sequence of microRNAs is often site of SNPs, so that in some patients the regulation of the targets can be disrupted/activated differently. Last and not least NGS is noticing that it does not exist one microRNA but several variants of the same with diverse profile of activity in some cases. Thus the simple dosing of one microRNA is not so informative as the microRNA-seq of the same sample.
Looking at the clinical applications for the above mentioned reasons the single dosing of a microRNA will never have in most of the cases an accuracy robust enough to warrant clinical use.
I agree with your opinion: the variation of a single microRNA could not explain the pathophysiology of a disease, nevertheless analysis of several circulating microRNAs (and comparison between patients with different disease progression or ageing related pathologies, i.e. neurological diseases) could be helpful in our comprehension of the pathological pathways involved, be exploitable as potential biomarkers and may lead to some novel therapeutic approach.
Some useful hints could derive from:
http://www.frontiersin.org/Journal/10.3389/fncel.2013.00150/abstract
Here you go
(https://www.researchgate.net/publication/51974434_Promising_Candidate_Urinary_MicroRNA_Biomarkers_for_the_Early_Detection_of_Hepatocellular_Carcinoma_among_High-Risk_Hepatitis_C_Virus_Egyptian_Patients?ev=prf_pub).
Enjoy
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