I would like to know more about the impact of freezing and thawing of cancer cell lines.
@ Christian usually the changes in the physico-chemical factor alter the signalling and stress condition which eventually provide a stress signal and aggravate protein-protein interaction in the cancer cell.
Here i am attaching links..
http://www.nature.com/ncb/journal/v8/n11/full/ncb1497.html
http://www.clinsci.org/cs/108/0369/1080369.pdf
I doubt that this is really relevant, since it is expected that under normal cell culture conditions tumor cells mutate. Thawing could disturb the already disturbed growth of tumor cell lines, but I am not aware that thawing generally causes a problem. Many cell lines and tumor cell lines change their behaviour and select for mutations, for example in p53, if not originally present in the tumor.
I don't think that freezing and thawing causes mutations. It can demage the cell membranes and kill the cells, but no more.
Freezing and thawing can kill cell then, then it can also cause mutation. If we see that the process of incomplete cell death may some time cause mutation.
Freezing and thawing can disturb the cell mass of cell line and also provide a stress signal to the concerning cell, which can alter the mitogenic signalling and can also bring mutation. But this is rare phenomena.
Secondly the most well suited answer is the alteration in temperature like often freezing brings an regular simulation for cancer cell signalling and resulted in cellular mutation.
I agree that thaw and freeze can only disturb cell membrane permeability but could not effect on DNA integrity.
@Bibekananda: The cells usually die because damages of the cell membranes by ice crystalls.
@Arvind: What kind of signalling, which is similiar to cancer cells, is initiated by freezing?
@ Christian usually the changes in the physico-chemical factor alter the signalling and stress condition which eventually provide a stress signal and aggravate protein-protein interaction in the cancer cell.
Here i am attaching links..
http://www.nature.com/ncb/journal/v8/n11/full/ncb1497.html
http://www.clinsci.org/cs/108/0369/1080369.pdf
Rohan, do you have any Refs or data whioch proves your statement "It is very well known that gene expression will undergo a drastic change under freezing and thawing conditions" ?? I agree with those who say that freeze-thawing can damage membranes or kill cells. However, even if one does the freeze-thaw correctly-there is always some cell death when you thaw and start new passage. If you use 20% FBS in the freezing medium it helps minimize this cell death. Hope this
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