08 March 2022 1 3K Report

This is from an archaeological deposit on a floodplain, and I'm wondering if it's vivianite? It doesn't have the usual structure to it. The photo is at 400x magnification in PPL.

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Using a ZymoPURE II Plasmid kit, what could cause clarified lysate to turn pink and cloudy after binding buffer addition, and result in low yield?

Hi all, I used a ZymoPURE II Plasmid Maxiprep kit to isolate plasmid DNA and ended up with very low yield and poor quality. Specifically, less than 10ng/uL in 500uL from 150mL of culture, and...

15 May 2024 6,870 2 View

What short-term risks and long-term consequences could ensue if the challenges confronting the ERBTC remain unaddressed?

This question is in relation to the journal written by B.M. Kavuvalu and others entitled "Challenges facing blood transfusion services at a regional blood transfusion center in Western Kenya"....

11 April 2024 4,126 1 View

How does platelet contamination affect the measurement of Lactate Dehydrogenase (LDH) when it comes to lithium-heparinized plasma tubes?

This question is derived from the journal article “The Transition from Gel Separatory Serum to Lithium Heparin Gel Tubes in the Clinical Laboratory,” written by Oguzhan Zengi. The journal...

07 April 2024 1,838 1 View

Can someone point us to software to identify closely short related protein sequences in other proteins as compared to a target short protein sequence?

Can someone point us towards analysis software that is able to identify similar short protein sequences within a protein database through use of a set of comparisons (e.g., similar charged or...

25 February 2024 508 1 View

Why can I not elucidate a dose response in HepG2 cells during Simvastatin treatment?

I am working with HepG2 cells to observe effects of Simvastatin treatment on the cells. We are aiming to identify a dose response associated with levels of ATP, metabolic activity, and CoQ10...

29 November 2023 6,062 0 View

Software for oligo design for gene synthesis by Gibson method?

Is there any software that splits 300-400 DNA fragment into eight-twelve 60-mers-80mers for the synthesis of this fragment by Gibson method? Telesisbio synthesizes oligos for DNA fragment...

20 November 2023 7,714 0 View

Why are my HepG2 cells not growing when plated in a ninety six well plate?

I have been working with HepG2 cells for some time with no problem. The past couple months when I've been plating into 96 well plate for experiments I am noticing the cells are not healthy or...

27 October 2023 8,292 3 View

For Amplex Red, why does my negative control emit higher fluorescence than my treatment groups?

My groups are: (A) Krebs-Ringer phosphate buffer (no treatment/control) incubated with cells (B) Krebs-Ringer phosphate buffer (plus treatment - high glucose) incubated with cells (C) Krebs-Ringer...

20 September 2023 6,845 0 View

What is the cause of this crystal formation in an MTT assay that showed low cell viability?

I have done a number of MTT assay's for a study I am a part of, twice the results showed a very low viability and this crystal formation. I did a later assay following the same procedure, using...

29 July 2023 8,014 2 View

Crispr/cas12 detects cDNA?

Does anyone know if crispr/cas12 detects cDNA?

06 July 2023 6,746 0 View

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