Technical site help needed: I need to change my log-in email address and can't get to the HELP section?

My old log in email, [email protected], was compromised and I need to change it to [email protected], so I can change my password, update publications, etc.

24 December 2020 6,939 3 View

Which Universities are virtual?

Many Universities have employees located across the world.

22 December 2020 6,380 3 View

How do I test for multicollinearity among Independent variables in binary logistic regression using R?

Please, I am trying to do a project work using logistic regression. I need to test for multicollinearity for the independent variables using R. I installed mctest and multiColl package already....

18 December 2020 2,531 3 View

Where can I find graphs of Brazilian Portuguese phonology?

I need research material on Brazilian Portuguese phonology.

27 September 2020 9,274 3 View

How to avoid getting organic particles to charr(become black) during calcination?

I am trying to synthesize SrHfO3:Eu2+ nanoparticles. To reduce from Eu3+ to Eu2+, we calcinate it under Ar/H2 conditions but it ends giving a black coloured product. We need this for luminescence...

22 August 2020 448 3 View

How does one collect data from a population that was once together on a university campus but is now dispersed due to the Covid-19 lockdowns??

If a researcher is more than half way through a research project, and now needs to collect data by recording the responses of participants to a read aloud test; How can such a researcher collect...

03 August 2020 6,771 5 View

How can we calculate polystyrene nanoparticles concentration in a solution?

I wonder how to measure the concentration of polystyrene nanoparticles (50nm) in a solution. Our nanoparticles are not fluorescents. Thanks

28 October 2019 2,353 4 View

I need some information on the method of manufacturing dry extract?

i need some information on the method of manufacturing dry extract?

06 February 2018 5,565 2 View

How can I export my publications as a Bib TeX file

I want to post my publications on ORCID and I can't see how to save them as a Bib TeX file in order to export them, as the instructions on ORCID say.

29 June 2017 6,242 2 View

Is it possible to cut a band from an agarose gel and purify it for subsequently creating a stock solution for qPCR?

09 May 2017 3,459 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View