Hi, guys. I think I met a problem now because my supervisor asked me to change from ELISA into Mouse,Inflammation,Kit,RUO - 552364 to test several cytokine at the same time. Could you please help me in the protocol because I cannot understand the protocol. In addition, I cannot find the software in FACS during this process because they need speical software for FACS analysis as the ELISA. Could you please help us?
https://www.irepertoire.com/services?gclid=CjwKCAjwo87YBRBgEiwAI1LkqcP3-8LfNpXJtp4ZP1kTxMeMviW_iTCmnGQWlJMzI2cAdpT-OIHpthoCzKwQAvD_BwE
You need to talk to your advisor, this kit was designed for the BD machines, and was optimized using them. This is why in the protocol they give you a list of the machines and what the protocols are needed to use this kit.
So... unless you have access to one of the BD flow listed in the protocol, I would NOT use this kit.
As for the flow analysis program, yes it does require the special software, because the program take the MFI and generate a standard curve and calculate the concentration based on that. Usually, this is not something a regular flow program does. Granted, you can modify it to do that, but this would require you knowing how to operate a normal flow analysis program.
I know this doesn't answer your question. I would suggest you talk to the flow operator, or whoever runs your flow lab for help.
Hi Shuoyang. The product you have mentioned is from BD Biosciences. You need access to a flow cytometer for the same. For analysis, software is FCAP array, which needs FCS 2.0 or higher version files. I hope that will be helpful for you.
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