Shuoyang Liu

6 Questions 2 Answers 0 Followers

Questions related from Shuoyang Liu

What is the relationship between LPS type and LPS-induced Treg expansion in sepsis ?

Dear friends, Recently, we use LPS to induce 24h-mouse spesis model to explore the effect of some drugs to splenic Treg expansion. However, sometimes we failed to induce sepsis and splenic Treg...

13 March 2019 3,010 1 View

May I know what is the softare for cytokine analysis by mouse inflammation kit?

Hi, guys. I think I met a problem now because my supervisor asked me to change from ELISA into Mouse,Inflammation,Kit,RUO - 552364 to test several cytokine at the same time. Could you please...

02 June 2018 9,567 3 View

What is the viability of cultured MACS-sorted mouse CD4+ T cells from spleen and LNs in RPMI1640 without IL-2 or anti-cd3/28 after 24h?

Hi, guys. I want to use MACS-sorted mouse CD4+ T cells from spleen and LNs to test the effect of an drug and some directors asked me to culture T cells without IL-2/CD3/28 to exclude the effect of...

28 March 2018 9,213 2 View

How long can I culture MACS-sorted mouse CD4+ T cells in RPMI1640 without IL-2 or anti-cd3/28?

Hi, guys. I want to use MACS-sorted mouse CD4+ T cells to test the effect of an drug and some directors asked me to culture T cells without IL-2/CD3/28 to exclude the effect of these stimulation....

26 March 2018 186 7 View

The problem of FACS-sorting

Hi, guys: I am sorting Tregs cells from the spleen and LNs of Foxp3-GFP KI mice now. In fact, I met some problems recent: first, the CD4+ Foxp3+ Cells are gated above lymphocytes...

29 July 2017 6,166 1 View

DHX analyzer in mice blood and spleen analysis.

Could I please ask if DxH500 analyzer can analyze mouse blood cells (and splenic cells and lymphoid cells)？

11 March 2017 1,058 0 View