Hello, I would like to knock-in a tag at N-term. into my gene of interest. The strategy is to clone 1.promoter, 2. gene and 3. 3'UTR into the plasmid and then transform. Now the difficulty begins, I have a very very long gene with many small exons and introns, which is difficult to amplify. Are there any moss people here, who can will agree if I can clone cDNA instead of the whole gene and if in such case it would still be a Knockin?!

Thanks