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Hello, I am measuring stomatal index in WT and mutant arabidopsis plant. The formula for SI says to divide the stomatal number by total number of stomata+epidermal cells. I am bit confused on how...
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Hello specialist in stomatas, I would like to analyze the stomata at various stage of ABA and/or flg treatments. So I was wondering if its possible to fix the leaf samples and then analyze them...
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Hello, I would like to remove selection cassette from my mutant plant in order to transform another construct with same selection. I know, cre/lox recombination is one of the possibility, but...
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Hello, I would like to knock-in a tag at N-term. into my gene of interest. The strategy is to clone 1.promoter, 2. gene and 3. 3'UTR into the plasmid and then transform. Now the difficulty begins,...
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Needs some expert advice in purifying a 10,300 kb fragment (after restriction) from agarose gel? I am getting very low yield, only few ngs!!! whereas my starting concentrations is more than 5 ugs....
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