Macrophage cells isolation?

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About forging of Mo Alloys ?

Hello dear Academicians, I am working on the forging of Mo alloys, but I wanted to consult you on an issue I could not solve. Mo is easily oxidized during forging and MoO3 is formed. Is it...

09 February 2021 1,989 3 View

What are the applicable reinforcement learning based control methods for systems that have control delay?

I am studying the experimental application of reinforcement learning (RL) based control methods. For that reason, I modeled a nonlinear system without delay and trained the RL agent. In this...

02 February 2021 7,999 3 View

How to get the insoluble fraction of PK digestion for mitochondria?

I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...

27 January 2021 5,145 2 View

How can I produce Alpha-monopropionin and Alpha-monobutyrin in lab scale?

I understand that glycerol should be reacted with propaoiocic acid to produce monopropionin and with butyric acid to produce monobutyrin , if you can help with the reaction conditions based on 1...

13 January 2021 1,151 4 View

Molydenum Alloys Forging Process ?

Hello dear Academicians, I've been struggling on a specific subject for a while. Hence, I'd like to get some advice. I am working on Mo-0.5 Ti alloy production via powder metallurgy technic. I...

20 December 2020 3,805 2 View

What is the appropriate period for indexing an article in the Scopus database?

Dear RG members The indexing of a published article in the Scopus database may depend on several factors. The issue is that my colleague and I have published separately two articles in the same...

16 December 2020 4,361 19 View

What is the reason vacuum performance degradation of the AAS analyser?

I'm working with Agilent 200 series atomic absorption spectroscopy in the lab. There is no problem when I first started the analysis. As time passes, for example half an hour after starting the...

01 December 2020 3,867 3 View

I need equations to calculate the activity of the enzyme glutamanase and basic phosphatase in soil and plants?

I need the rates for calculating the mentioned enzymes after the laboratory work. I have trouble calculating and extracting the activity of the mentioned enzymes in soil and plants because of the...

03 November 2020 8,601 1 View

How can I know that a variant is novel?

I found a variant which is not reported in gnomAD or ExAC, but still has an rs. Should I consider it as a novel variant? when can I say that ceratin variant is novel?

15 October 2020 9,539 3 View

Do you have any qualitative or quantitative methodologies for evaluating gender equality plans in HE?

I am collating evaluation methodologies. So, broader methodologies for inclusion in my report are also welcome. Please also indicate the preferred citation for the evaluation methodology.

14 October 2020 4,266 7 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Macrophages in culture not growing/ dying or detaching . Why ?

Hello, I am an undergraduate student doing my dissertation, where I isolate PBMCs from leucocyte cones in order to plate monocytes and allow for macrophage differentiation. Although the numbers...

09 February 2021 5,334 8 View

Rat Macrophage Markers for Flow Cytometry?

Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.

04 February 2021 3,913 4 View

Can anyone help me with doing resorption pit assay on biomaterials for 28 days?

I culture RAW 264.7 cells on my samples and differentiate them to osteoclasts with RANKL. I want to do resorption pit assay by culturing cells for 28 days. My problem is that the macrophages keep...

01 February 2021 6,746 2 View

What M1 and M2 markers can I use for flow cytometry ?

Hi, I'm a student and I'm doing an internship. I need to asses the M1 and M2 populations of a culture using flow cytometry. From my researches, I know that this is subject to some controversy, as...

26 January 2021 299 3 View

What are the validation parameters for RAW BLUE macrophages 264.7?

Currently, I am working with RAW BLUE cell lines to check the SEAP expression on addition of TLR ligands? Method developed for different TLR ligands. How experiments can design for validations?...

24 January 2021 589 2 View

Can I use 24 wells black tissue culture uncoated plate for cultuing macrophage (THP-1 treated with PMA) ?or I have to coat them?

Hello, I will start an experiment using macrophage (THP-1 treated with PMA). I have ordered the plate that suitable for fluorescence plate reader which is glass 24 wells black uncoated tissue...

05 January 2021 4,214 3 View

No title

Hi, I am interested to see the effect of BCG on macrophages (microglia )Therefore , I would like to know the amounts and the routes of administration which is safe in mice and how long is...

20 December 2020 7,166 2 View

Has anybody had staining problems with lysotracker in live cells?

I am working with two fish cells types, a zebrafish liver cell line and primary cultures of trout macrophages. We are using lysotracker to stain them in order to visualize lysosomes in confocal...

16 December 2020 3,935 2 View

My Wright-Giemsa Stain on macrophages isn't showing any cytoplasm, just nucleus. Any tips to improve my staining?

I'm pretty sure they are mostly macrophages since after a 2 hour incubation in M199 they are stuck with pseudopodia. My slides just aren't pretty enough for me. I'm using Fisherbrand Wight-Giemsa...

13 December 2020 5,475 3 View

Ido From

https://www.stemcell.com/how-to-use-sepmate-to-isolate-pbmcs-from-whole-blood-in-just-15-minutes.html?utm_source=google&utm_medium=cpc&gclid=EAIaIQobChMIq5aDp6vT-wIVFRwGAB1r6AD6EAAYAyAAEgJnDPD_BwE

James E Talmadge

Monocytes can be isolated from PB but need to be differentiated/activated into macrophages. Relevant questions include, what you plan on using the macrophages for and how many you need? The answers to these questions will drive the most appropriate approach.

Mustafa Selim Doğru

Dear Ido From, thanks for your interest.

Dear James E Talmadge, thank you for your attention. I am planning to determine the effect of a drug on cytokine levels on activated macrophages.