I read a paper from PNAS (http://www.pnas.org/content/101/46/16222). They used a lysis buffer with high amount of PVP (6,3g/5mL). It is very viscous, I can't take it by pipet!!!

To each gram of separated cells or whole-sponge tissue ground in liquid nitrogen was added 5 ml of lysis buffer containing 100 mM Tris·HCl (pH 8), 1.4 M NaCl, 20 mM EDTA, 2 ml of CTAB solution at 55°C, 100 μl of 10% SDS, 350 μl of 100 mM diethyldithiocarbamate (DETC), 100 μl of mercaptoethanol, 6.3 g of polyvinylpyrrolidone, 10 mg of lysozyme, and 500 μg of proteinase K.