I will be taking surface samples with a cotton tip swab that will have a low biomass amount. I need to extract and purify the DNA which will likely have low amounts of microorganisms. I will be working to optimize the protocol and am currently working with Claremont biosolutions reagents. I was wondering if there is any recommendations to use a mixture or lysozyme and mutanolysin? If anyone has experience with this type of experiment it would be greatly appreciated.
Update: The sample is going to contain a mixture of bacteria which will be obtained from an area where human traffic is present. Also will not be able to culture the organism O/N because we are attempting to identify what is present as soon as possible after the swab has been collected.
Thank you
You didn't specify what bacteria you are using. Generally enzymes that break the cell wall i.e. lysostaphin, etc help a lot in the DNA extraction from gram positive bacteria S. aureus for example. And why not to grow an overnight culture of the organism then use it to extract the DNA I found this to make a big difference.
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