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I generated S. aureus mutants by transduction and then complemented the mutants with the wild type alleles using a shuttle recombinant vector. the problem is when I done broth micro-dilution...
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Hi anyone could help with a protocol to prepare competent cells from S. aureus. I used several of them unfortunately didn't work for me. I am trying to complement mutants I generated previously by...
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I use this kit trying to clone a gene 1011 bp in size into a shuttle vector 6000 bp. I got transformants on the plates but after the screening using either sequencing or colony PCR nothing there...
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