Christian Castro

2 Questions 1 Answers 0 Followers

Questions related from Christian Castro

Long Read rDNA fungal read for speciation? ITS1/2 + flanking regions LSU/SSU?

Hello, I am looking to obtain a contig of atleast 1500 bp to identify fungi on the species level. Is there any publications or recomendations of primers to amplify the ITS region and the flanking...

04 November 2017 4,944 1 View

Low biomass samples and the most efficient way to extract and purify the DNA? Will a chemical mixture of lysozyme and protienase K work?

I will be taking surface samples with a cotton tip swab that will have a low biomass amount. I need to extract and purify the DNA which will likely have low amounts of microorganisms. I will be...

17 March 2017 7,251 1 View