What factors influence the growth of the informal economic sector?

Business in informality can be caused by factors such as the facilitating business environment, tax rates, forms of consumption by buyers, lack of administrative knowledge, complexity of the...

01 January 2021 7,189 5 View

If an observed d falls beyond the upper limit in equivalence testing, what does that mean?

I have been taking your Coursera Classes and reading some of your articles on equivalence testing, and I do not know how to interpret and effect and its corresponding CI, but I do not how to...

12 October 2020 9,046 5 View

[Energies] Special Issue - Wind, Solar, Load, and Electricity Price Forecasting ?

Dear Colleagues, I am pleased to announce that I was invited as a Guest Editor for a special issue on the Energies journal. The open-access journal Energies (ISSN 1996-1073, IF 2.702) is...

26 July 2020 4,028 4 View

Is valid this complexity theory technique to solve outstanding problems?

04 June 2020 7,203 2 View

Hello Who can help me with this error of hec hms ERROR 41742: Invalid initial loss for subbasin??

This error apears only in subasins with a value superior than 19, 682 in the initial abstraction in the method SCS Number Curve.

18 May 2020 6,985 3 View

Coefficient of variation?

Hi, everyone. What's the different, if any, between calculating the coefficient of variation of a group of data disposed in a experimental design with different treatments (for ANOVA) and a "free"...

20 March 2020 4,871 3 View

Are they valid these complexity theory techniques to solve outstanding problem?

03 March 2020 1,035 5 View

Low 260/230 ratio using Qiagen DNeasy kit?

Recently I performed a DNA extraction using Qiagen DNeasy kit and the results show a 260/230 ratio of 1.05 likely due the presence of phenol or other organic contaminant... I have read that you...

16 February 2020 787 3 View

Please, how to run a Cross-lagged Panel Model in Stata?

I am using panel data to search for the causality between two variables, and I think a Cross-lagged Panel Model would be appropriate. I have 8 waves and I want to run de model wave by wave in...

10 November 2019 9,736 4 View

How to growth spirulina in 1m3 tank?

I want to try different methodologies to grow spirulina in a 1 cubic meter tank and compare the production yield. Please if you have a tip, let me know.

13 September 2019 1,583 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View